干旱区生态环境知识资源中心(Arid): Development of sorghum mutants with improved in vitro protein digestibility by CRISPR/Cas9 editing of kafirin genes

Arid

DOI	10.1016/j.cj.2023.02.005
	Development of sorghum mutants with improved in vitro protein digestibility by CRISPR/Cas9 editing of kafirin genes
	Elkonin, Lev A.; Gerashchenkov, Grigoriy A.; Borisenko, Natalie, V; Kenzhegulov, Odyssey A.; Sarsenova, Saule Kh.; Rozhnova, Natalya A.; Panin, Valery M.
通讯作者	Elkonin, LA
来源期刊	CROP JOURNAL
ISSN	2095-5421
EISSN	2214-5141
出版年	2023
卷号	11 期号:5 页码:1411-1418
英文摘要	Sorghum (Sorghum bicolor (L.) Moench) is a major world crop that is a reliable source of fodder and food grain in arid regions. However, unlike other cereals, sorghum grain has low nutritional value, owing mainly to the resistance of its storage proteins (kafirins) to protease digestion. Changing the composition of kafirins or their primary structure may address this problem. To induce mutations in kafirin-encoding genes that were expected to disturb their accumulation in endosperm cells, we used a genome-editing approach. By Agrobacterium-mediated genetic transformation of immature embryos of cv. Avans, we obtained 14 transgenic plants with genetic constructs for site-directed mutagenesis of the k1C5 and gKAF1 genes encoding 22 kDa a- and 28 kDa c-kafirins, respectively. Sequencing of 5 regenerants obtained by using k1C5-addressing vector revealed two plants with mutations. T1 progeny of these mutants had higher in vitro digestibility of endosperm proteins (86%-92%), in comparison with the donor Avans (63%-67%). The kernels of these plants had a thick vitreous endosperm. A mutant with increased in vitro protein digestibility and vitreous endosperm, carrying a mutation in the target sequence, was also obtained by use of the gKAF1-addressing vector. Thus, using genome editing technology, we have obtained mutants with improved kafirin digestibility that can be used in sorghum breeding.(c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
英文关键词	Sorghum CRISPR/Cas Kafirins In vitro protein digestibility Vitreous endosperm
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:001089621600001
WOS关键词	STARCH DIGESTIBILITY ; TRANSGENIC SORGHUM ; STORAGE PROTEINS ; COOKED SORGHUM ; GRAIN ; VITREOUSNESS ; QUALITY ; BICOLOR
WOS类目	Agronomy ; Plant Sciences
WOS研究方向	Agriculture ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/395831
推荐引用方式 GB/T 7714	Elkonin, Lev A.,Gerashchenkov, Grigoriy A.,Borisenko, Natalie, V,et al. Development of sorghum mutants with improved in vitro protein digestibility by CRISPR/Cas9 editing of kafirin genes[J],2023,11(5):1411-1418.
APA	Elkonin, Lev A..,Gerashchenkov, Grigoriy A..,Borisenko, Natalie, V.,Kenzhegulov, Odyssey A..,Sarsenova, Saule Kh..,...&Panin, Valery M..(2023).Development of sorghum mutants with improved in vitro protein digestibility by CRISPR/Cas9 editing of kafirin genes.CROP JOURNAL,11(5),1411-1418.
MLA	Elkonin, Lev A.,et al."Development of sorghum mutants with improved in vitro protein digestibility by CRISPR/Cas9 editing of kafirin genes".CROP JOURNAL 11.5(2023):1411-1418.