干旱区生态环境知识资源中心(Arid): Overexpression of ZxABCG11 from Zygophyllum xanthoxylum enhances tolerance to drought and heat in alfalfa by increasing cuticular wax deposition

Arid

DOI	10.1016/j.cj.2022.11.007
	Overexpression of ZxABCG11 from Zygophyllum xanthoxylum enhances tolerance to drought and heat in alfalfa by increasing cuticular wax deposition
	Liu, Linbo; Bao, Aike; Li, Hujun; Bai, Wanpeng; Liu, Haishuang; Tian, Ye; Zhao, Yiying; Xia, Fangchen; Wang, Suomin
通讯作者	Bao, AK ; Wang, SM
来源期刊	CROP JOURNAL
ISSN	2095-5421
EISSN	2214-5141
出版年	2023
卷号	11 期号:4 页码:1140-1151
英文摘要	Drought and heat stresses cause yield losses in alfalfa, a forage crop cultivated worldwide. Improving its drought and heat tolerance is desirable for maintaining alfalfa productivity in hot, arid regions. Cuticular wax forms a protective barrier on aerial surfaces of land plants against environmental stresses. ABCG11 encodes an ATP binding cassette (ABC) transporter that functions in the cuticular wax transport pathway. In this study, ZxABCG11 from the xerophyte Zygophyllum xanthoxylum was introduced into alfalfa by Agrobacterium tumefaciens-mediated transformation. Compared to the wild type (WT), transgenic alfalfa displayed faster growth, higher wax crystal density, and thicker cuticle on leaves under normal condition. Under either drought or heat treatment in greenhouse conditions, the plant height and shoot biomass of transgenic lines were significantly higher than those of the WT. Transgenic alfalfa showed excellent growth and 50% greater hay yield than WT under field conditions in a hot, arid region. Overexpression of ZxABCG11 up-regulated wax-related genes and resulted in more cuticular wax deposition, which con-tributed to reduction of cuticle permeability and thus increased water retention and photosynthesis capacity of transgenic alfalfa. Thus, overexpression of ZxABCG11 can simultaneously improve biomass yield, drought and heat tolerance in alfalfa by increasing cuticular wax deposition. Our study provides a promising avenue for developing novel forage cultivars suitable for planting in hot, arid, marginal lands.(c) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
英文关键词	Alfalfa ZxABCG11 Cuticular wax Yield Stress tolerance
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:001081356000001
WOS关键词	ABC TRANSPORTER GENE ; CUTICLE LIPIDS ; BIOSYNTHESIS ; WATER ; ACCUMULATION ; TEMPERATURE ; ECERIFERUM3 ; EXPRESSION ; ENCODES ; STRESS
WOS类目	Agronomy ; Plant Sciences
WOS研究方向	Agriculture ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/395830
推荐引用方式 GB/T 7714	Liu, Linbo,Bao, Aike,Li, Hujun,et al. Overexpression of ZxABCG11 from Zygophyllum xanthoxylum enhances tolerance to drought and heat in alfalfa by increasing cuticular wax deposition[J],2023,11(4):1140-1151.
APA	Liu, Linbo.,Bao, Aike.,Li, Hujun.,Bai, Wanpeng.,Liu, Haishuang.,...&Wang, Suomin.(2023).Overexpression of ZxABCG11 from Zygophyllum xanthoxylum enhances tolerance to drought and heat in alfalfa by increasing cuticular wax deposition.CROP JOURNAL,11(4),1140-1151.
MLA	Liu, Linbo,et al."Overexpression of ZxABCG11 from Zygophyllum xanthoxylum enhances tolerance to drought and heat in alfalfa by increasing cuticular wax deposition".CROP JOURNAL 11.4(2023):1140-1151.