干旱区生态环境知识资源中心(Arid): Gender identification in Siirt pistachio (Pistacia vera L. cv. Siirt) trees and saplings using the SCAR marker

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DOI	10.1007/s40415-023-00917-5
	Gender identification in Siirt pistachio (Pistacia vera L. cv. Siirt) trees and saplings using the SCAR marker
	Ersali, Yusuf; Inal, Behcet; Sezgin, Naci
通讯作者	Ersali, Y
来源期刊	BRAZILIAN JOURNAL OF BOTANY
ISSN	0100-8404
EISSN	1806-9959
出版年	2023
卷号	46 期号:4 页码:907-912
英文摘要	The Pistacia genus has become widely spread in the area where other cultivated plants do not grow on sloped, stony, or rocky lands and in arid/semi-arid regions of Northern Africa, the Middle East, the Mediterranean of Southern Europe, and Western and Central Asia. The members of this genus are dioecious in nature. Gender identification in Pistacia species is economically important for pistachio producers. Crop production and gains can be delayed because of the long juvenile period ranging from five to ten years. Some morphological, biochemical, and molecular techniques are available to learn the gender of saplings of this genus. Molecular markers, however, are expected to enable a method that helps in gender identification at the juvenile stage. Fragments obtained from randomly amplified polymorphic DNA (RAPD) were cloned, and sequence-characterized amplified region (SCAR) markers with strong distinguishing properties were developed. In the current study, four primers (OPL11, BC156, BC360, and OPAK09) were tested, but only the OPL11 primer formed a reproducible band (about 261 base pairs) in female pistachios. The distinguishing RAPD band of the OPL11 primer was purified from the gel, cloned, and sequenced for female pistachio trees. The specific primers were synthesized for the amplification of about a 261-base pair fragment in female pistachio trees. The designed SCAR markers were applied to distinguish female and male trees and saplings of the pistachio plant, but specific bands with expected sizes were found only in female trees and some saplings of pistachio plants.
英文关键词	Gender Marker Primer Pistachio Sapling
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001051846900001
WOS关键词	SEX DETERMINATION ; PCR
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/395614
推荐引用方式 GB/T 7714	Ersali, Yusuf,Inal, Behcet,Sezgin, Naci. Gender identification in Siirt pistachio (Pistacia vera L. cv. Siirt) trees and saplings using the SCAR marker[J],2023,46(4):907-912.
APA	Ersali, Yusuf,Inal, Behcet,&Sezgin, Naci.(2023).Gender identification in Siirt pistachio (Pistacia vera L. cv. Siirt) trees and saplings using the SCAR marker.BRAZILIAN JOURNAL OF BOTANY,46(4),907-912.
MLA	Ersali, Yusuf,et al."Gender identification in Siirt pistachio (Pistacia vera L. cv. Siirt) trees and saplings using the SCAR marker".BRAZILIAN JOURNAL OF BOTANY 46.4(2023):907-912.