Arid
DOI10.1186/s12917-023-03709-x
Analysis of the stability of the reference genes GAPDH, SDHA and RPL-19 in sheep from a semi-arid region infected by gastrointestinal nematodes
Souza, Jorge Lucas Nascimento; Silva, Fernanda Cavalcante; da Silva, Carlikelly Gleicy; Bomfim, Isabela Maria Fortaleza Neves; de Medeiros, Henrique Rocha; Zaros, Lilian Giotto
通讯作者Zaros, LG
来源期刊BMC VETERINARY RESEARCH
EISSN1746-6148
出版年2023
卷号19期号:1
英文摘要Analyzing the stability of reference genes already described as universal is an important methodology to lead gene expression analysis because different studies have shown that the expression of universal reference genes may vary between experimental treatments. In this sense, the glyceraldehyde 3-phosphate dehydrogenase (GAPDH), Succinate dehydrogenase complex subunit A (SDHA) and Ribosomal Protein L-19 (RPL-19) reference genes (already described in other studies with sheep from different regions, breeds and infectious agents or in organisms evolutionarily close to sheep) were investigated in the abomasum, small and large intestines of resistant and susceptible crossbred sheep groups to gastrointestinal nematode infections in the Semi-arid region in Northeast of Brazil. The animals were naturally infected to determine the resistance or susceptibility status by counting eggs per gram (EPG) of feces from the gastrointestinal tract after 33 weeks of observations of infection evolution. Relative gene expression was performed by RT-qPCR methodology using Sybr green and relative gene expression stability was tested by different software programs such as REST, BestKeeper, geNorm and Normfinder. Our results showed the susceptible animals had increase in egg counts per gram of feces than resistant animals (p < 0.001), and both groups showed a mixed infection by nematodes of the genus Haemonchus, Trichostrongylus, Oesophagostomum and Trichuris. Furthermore, we show the importance of analyzing different genes in different software programs and the importance to choose ideal reference genes. In this sense, GAPDH was the most stable gene in the abomasum, whereas SDHA was the most stable in the small and large intestines. In addition, we discuss about variables which can interfere in relative expression such as breed, species, climate and tissue. However, utilizing other reference genes already described in other studies with the same and different variables should be performed.
英文关键词GAPDH Gastrointestinal tract Sheep and RT-qPCR
类型Article
语种英语
开放获取类型gold
收录类别SCI-E
WOS记录号WOS:001064981600003
WOS关键词TIME QUANTITATIVE PCR ; HAEMONCHUS-CONTORTUS ; EXPRESSION ANALYSIS ; RT-PCR ; RESISTANCE ; MECHANISMS ; IVERMECTIN ; SELECTION ; COOPERIA ; HISTORY
WOS类目Veterinary Sciences
WOS研究方向Veterinary Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/395597
推荐引用方式
GB/T 7714
Souza, Jorge Lucas Nascimento,Silva, Fernanda Cavalcante,da Silva, Carlikelly Gleicy,et al. Analysis of the stability of the reference genes GAPDH, SDHA and RPL-19 in sheep from a semi-arid region infected by gastrointestinal nematodes[J],2023,19(1).
APA Souza, Jorge Lucas Nascimento,Silva, Fernanda Cavalcante,da Silva, Carlikelly Gleicy,Bomfim, Isabela Maria Fortaleza Neves,de Medeiros, Henrique Rocha,&Zaros, Lilian Giotto.(2023).Analysis of the stability of the reference genes GAPDH, SDHA and RPL-19 in sheep from a semi-arid region infected by gastrointestinal nematodes.BMC VETERINARY RESEARCH,19(1).
MLA Souza, Jorge Lucas Nascimento,et al."Analysis of the stability of the reference genes GAPDH, SDHA and RPL-19 in sheep from a semi-arid region infected by gastrointestinal nematodes".BMC VETERINARY RESEARCH 19.1(2023).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Souza, Jorge Lucas Nascimento]的文章
[Silva, Fernanda Cavalcante]的文章
[da Silva, Carlikelly Gleicy]的文章
百度学术
百度学术中相似的文章
[Souza, Jorge Lucas Nascimento]的文章
[Silva, Fernanda Cavalcante]的文章
[da Silva, Carlikelly Gleicy]的文章
必应学术
必应学术中相似的文章
[Souza, Jorge Lucas Nascimento]的文章
[Silva, Fernanda Cavalcante]的文章
[da Silva, Carlikelly Gleicy]的文章
相关权益政策
暂无数据
收藏/分享

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。