干旱区生态环境知识资源中心(Arid): Genetic diversity, population structure, and genome-wide association study for the flowering trait in a diverse panel of 428 moth bean (Vigna aconitifolia) accessions using genotyping by sequencing

Arid

DOI	10.1186/s12870-023-04215-w
	Genetic diversity, population structure, and genome-wide association study for the flowering trait in a diverse panel of 428 moth bean (Vigna aconitifolia) accessions using genotyping by sequencing
	Yadav, Arvind Kumar; Singh, Chandan Kumar; Kalia, Rajwant K.; Mittal, Shikha; Wankhede, Dhammaprakash P.; Kakani, Rajesh K.; Ujjainwal, Shraddha; Aakash; Saroha, Ankit; Nathawat, N. S.; Rani, Reena; Panchariya, Pooja; Choudhary, Manoj; Solanki, Kantilal; Chaturvedi, K. K.; Archak, Sunil; Singh, Kuldeep; Singh, Gyanendra Pratap; Singh, Amit Kumar
通讯作者	Wankhede, DP ; Singh, AK
来源期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
出版年	2023
卷号	23 期号:1
英文摘要	BackgroundMoth bean (Vigna aconitifolia) is an underutilized, protein-rich legume that is grown in arid and semi-arid areas of south Asia and is highly resistant to abiotic stresses such as heat and drought. Despite its economic importance, the crop remains unexplored at the genomic level for genetic diversity and trait mapping studies. To date, there is no report of SNP marker discovery and association mapping of any trait in this crop. Therefore, this study aimed to dissect the genetic diversity, population structure and marker-trait association for the flowering trait in a diversity panel of 428 moth bean accessions using genotyping by sequencing (GBS) approach.ResultsA total of 9078 high-quality single nucleotide polymorphisms (SNPs) were discovered by genotyping of 428 moth bean accessions. Model-based structure analysis and PCA grouped the moth bean accessions into two subpopulations. Cluster analysis revealed accessions belonging to the Northwestern region of India had higher variability than accessions from the other regions suggesting that this region represents its center of diversity. AMOVA revealed more variations within individuals (74%) and among the individuals (24%) than among the populations (2%). Marker-trait association analysis using seven multi-locus models including mrMLM, FASTmrEMMA FASTmrEMMA, ISIS EM-BLASSO, MLMM, BLINK and FarmCPU revealed 29 potential genomic regions for the trait days to 50% flowering, which were consistently detected in three or more models. Analysis of the allelic effect of the major genomic regions explaining phenotypic variance of more than 10% and those detected in at least 2 environments showed 4 genomic regions with significant phenotypic effect on this trait. Further, we also analyzed genetic relationships among the Vigna species using SNP markers. The genomic localization of moth bean SNPs on genomes of closely related Vigna species demonstrated that maximum numbers of SNPs were getting localized on Vigna mungo. This suggested that the moth bean is most closely related to V. mungo.ConclusionOur study shows that the north-western regions of India represent the center of diversity of the moth bean. Further, the study revealed flowering-related genomic regions/candidate genes which can be potentially exploited in breeding programs to develop early-maturity moth bean varieties.
英文关键词	Flowering time GBS GWAS Multi-locus association Moth bean SNPs
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000978482900001
WOS关键词	PECTIN ESTERASE ; DOMESTICATION ; SOFTWARE ; ACTIN ; TIME ; RESISTANCE ; STACKS ; POLLEN
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/395590
推荐引用方式 GB/T 7714	Yadav, Arvind Kumar,Singh, Chandan Kumar,Kalia, Rajwant K.,et al. Genetic diversity, population structure, and genome-wide association study for the flowering trait in a diverse panel of 428 moth bean (Vigna aconitifolia) accessions using genotyping by sequencing[J],2023,23(1).
APA	Yadav, Arvind Kumar.,Singh, Chandan Kumar.,Kalia, Rajwant K..,Mittal, Shikha.,Wankhede, Dhammaprakash P..,...&Singh, Amit Kumar.(2023).Genetic diversity, population structure, and genome-wide association study for the flowering trait in a diverse panel of 428 moth bean (Vigna aconitifolia) accessions using genotyping by sequencing.BMC PLANT BIOLOGY,23(1).
MLA	Yadav, Arvind Kumar,et al."Genetic diversity, population structure, and genome-wide association study for the flowering trait in a diverse panel of 428 moth bean (Vigna aconitifolia) accessions using genotyping by sequencing".BMC PLANT BIOLOGY 23.1(2023).