干旱区生态环境知识资源中心(Arid): Gene expression differences consistent with water loss reduction underlie desiccation tolerance of natural Drosophila populations

Arid

DOI	10.1186/s12915-023-01530-4
	Gene expression differences consistent with water loss reduction underlie desiccation tolerance of natural Drosophila populations
	Horvath, Vivien; Guirao-Rico, Sara; Salces-Ortiz, Judit; Rech, Gabriel E.; Green, Llewellyn; Aprea, Eugenio; Rodeghiero, Mirco; Anfora, Gianfranco; Gonzalez, Josefa
通讯作者	González, J
来源期刊	BMC BIOLOGY
EISSN	1741-7007
出版年	2023
卷号	21 期号:1
英文摘要	BackgroundClimate change is one of the main factors shaping the distribution and biodiversity of organisms, among others by greatly altering water availability, thus exposing species and ecosystems to harsh desiccation conditions. However, most of the studies so far have focused on the effects of increased temperature. Integrating transcriptomics and physiology is key to advancing our knowledge on how species cope with desiccation stress, and these studies are still best accomplished in model organisms.ResultsHere, we characterized the natural variation of European D. melanogaster populations across climate zones and found that strains from arid regions were similar or more tolerant to desiccation compared with strains from temperate regions. Tolerant and sensitive strains differed not only in their transcriptomic response to stress but also in their basal expression levels. We further showed that gene expression changes in tolerant strains correlated with their physiological response to desiccation stress and with their cuticular hydrocarbon composition, and functionally validated three of the candidate genes identified. Transposable elements, which are known to influence stress response across organisms, were not found to be enriched nearby differentially expressed genes. Finally, we identified several tRNA-derived small RNA fragments that differentially targeted genes in response to desiccation stress.ConclusionsOverall, our results showed that basal gene expression differences across individuals should be analyzed if we are to understand the genetic basis of differential stress survival. Moreover, tRNA-derived small RNA fragments appear to be relevant across stress responses and allow for the identification of stress-response genes not detected at the transcriptional level.
英文关键词	Cuticular hydrocarbons Water content Respiration rate Post-transcriptional regulation tRFs Insect physiology
类型	Article
语种	英语
开放获取类型	gold, Green Submitted, Green Published
收录类别	SCI-E
WOS记录号	WOS:000936861100003
WOS关键词	CUTICULAR HYDROCARBON PROFILE ; TRANSPOSABLE ELEMENTS ; CLIMATE-CHANGE ; STARVATION RESISTANCE ; FATTY-ACIDS ; MELANOGASTER ; STRESS ; RESPONSES ; EVOLUTION ; SELECTION
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/395582
推荐引用方式 GB/T 7714	Horvath, Vivien,Guirao-Rico, Sara,Salces-Ortiz, Judit,et al. Gene expression differences consistent with water loss reduction underlie desiccation tolerance of natural Drosophila populations[J],2023,21(1).
APA	Horvath, Vivien.,Guirao-Rico, Sara.,Salces-Ortiz, Judit.,Rech, Gabriel E..,Green, Llewellyn.,...&Gonzalez, Josefa.(2023).Gene expression differences consistent with water loss reduction underlie desiccation tolerance of natural Drosophila populations.BMC BIOLOGY,21(1).
MLA	Horvath, Vivien,et al."Gene expression differences consistent with water loss reduction underlie desiccation tolerance of natural Drosophila populations".BMC BIOLOGY 21.1(2023).