干旱区生态环境知识资源中心(Arid): Development and validation of an ultra-performance liquid chromatography-tandem mass spectrometry method for the determination of baloxavir in rat plasma and its application to pharmacokinetic studies

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DOI	10.1002/bmc.5729
	Development and validation of an ultra-performance liquid chromatography-tandem mass spectrometry method for the determination of baloxavir in rat plasma and its application to pharmacokinetic studies
	Mu, Hongli; Zhang, Wenyu; Song, Yuanming; Liang, Rui; Zhao, Hengli; Song, Chunhong; Wen, Qing
通讯作者	Wen, Q
来源期刊	BIOMEDICAL CHROMATOGRAPHY
ISSN	0269-3879
EISSN	1099-0801
出版年	2023
卷号	37 期号:11
英文摘要	In this study, an ultra-performance liquid chromatography-tandem mass spectrometry method was established for the development and validation of baloxavir acid (BXA) concentrations and the active ingredients of the antiviral drug baloxavir marboxil (BXM). Further, the method was applied to study the pharmacokinetics of BXA. BXA was determined by the electrospray ionization multiple reaction monitoring positive ion mode, and the mass-to-charge ratios (m/z) of BXA and internal standard baloxavir-d4 were 484.2 -> 247.2 and 488.1 -> 247.2. An Oasis max online column (2.1x20mm, 30 mu m) was used with 1% formic acid in water (A) and 2% formic acid in acetonitrile (B) as mobile phases at a flow rate of 0.5mL.min(-1) for chromatographic separation. The linearity was good in the range of 3-200ng.mL(-1) (r=0.9994), with 3.00ng.mL(-1) lower limit of quantification. The relative standard deviation of the inter-assay precision was less than or equal to 6.51%, and the accuracy was in the range of 91.28%-104.29%. This method is suitable for the determination of BXA and for performing pharmacokinetic studies in clinical research.
英文关键词	baloxavir marboxil pharmacokinetics UPLC-MS/MS
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001097558200013
WOS关键词	ARYLACETAMIDE DEACETYLASE ; MARBOXIL
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/395572
推荐引用方式 GB/T 7714	Mu, Hongli,Zhang, Wenyu,Song, Yuanming,et al. Development and validation of an ultra-performance liquid chromatography-tandem mass spectrometry method for the determination of baloxavir in rat plasma and its application to pharmacokinetic studies[J],2023,37(11).
APA	Mu, Hongli.,Zhang, Wenyu.,Song, Yuanming.,Liang, Rui.,Zhao, Hengli.,...&Wen, Qing.(2023).Development and validation of an ultra-performance liquid chromatography-tandem mass spectrometry method for the determination of baloxavir in rat plasma and its application to pharmacokinetic studies.BIOMEDICAL CHROMATOGRAPHY,37(11).
MLA	Mu, Hongli,et al."Development and validation of an ultra-performance liquid chromatography-tandem mass spectrometry method for the determination of baloxavir in rat plasma and its application to pharmacokinetic studies".BIOMEDICAL CHROMATOGRAPHY 37.11(2023).