干旱区生态环境知识资源中心(Arid): First Report of Mitochondrial DNA Copy Number Variation in Opsius heydeni (Insecta, Hemiptera, Cicadellidae) from Polluted and Control Sites

Arid

DOI	10.3390/ani13111793
	First Report of Mitochondrial DNA Copy Number Variation in Opsius heydeni (Insecta, Hemiptera, Cicadellidae) from Polluted and Control Sites
	Calogero, Giada Santa; Giuga, Marta; D'Urso, Vera; Ferrito, Venera; Pappalardo, Anna Maria
通讯作者	Ferrito, V ; Pappalardo, AM
来源期刊	ANIMALS
ISSN	2076-2615
出版年	2023
卷号	13 期号:11
英文摘要	Mitochondrial DNA easily undergoes alterations due to exposure to stress factors. In particular, mitochondrial DNA copy number (mtDNAcn) variation can be used as a biomarker of the effect of exposure to various environmental contaminants. In this study, a molecular investigation based on the evaluation of mtDNAcn variation was applied for the first time to individuals belonging to the species Opsius heydeni. A total of 20 samples were collected from two sites in eastern Sicily: Priolo Gargallo, a site with a strong anthropic impact, and the Simeto river Oasis, a control site. Specimens identified based on morphological traits were used to obtain COI gene sequences from this species that were not previously available in GenBank. After processing, the relative mtDNAcn was evaluated using real-time PCR of a portion of the COI and 18S genes. A decrease in the mtDNAcn in the specimens from the polluted site was observed. These results highlight how environmental contaminants can alter the mitochondrial genome and how Opsius heydeni can be considered a potential bioindicator species of environmental quality.
英文关键词	Opsius Insecta mtDNA copy number real-time PCR biomarker pollution
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:001004851000001
WOS关键词	ASSOCIATION ; BIOMARKER ; SALINITY ; SYSTEM ; DAMAGE
WOS类目	Agriculture, Dairy & Animal Science ; Veterinary Sciences ; Zoology
WOS研究方向	Agriculture ; Veterinary Sciences ; Zoology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/395306
推荐引用方式 GB/T 7714	Calogero, Giada Santa,Giuga, Marta,D'Urso, Vera,et al. First Report of Mitochondrial DNA Copy Number Variation in Opsius heydeni (Insecta, Hemiptera, Cicadellidae) from Polluted and Control Sites[J],2023,13(11).
APA	Calogero, Giada Santa,Giuga, Marta,D'Urso, Vera,Ferrito, Venera,&Pappalardo, Anna Maria.(2023).First Report of Mitochondrial DNA Copy Number Variation in Opsius heydeni (Insecta, Hemiptera, Cicadellidae) from Polluted and Control Sites.ANIMALS,13(11).
MLA	Calogero, Giada Santa,et al."First Report of Mitochondrial DNA Copy Number Variation in Opsius heydeni (Insecta, Hemiptera, Cicadellidae) from Polluted and Control Sites".ANIMALS 13.11(2023).