干旱区生态环境知识资源中心(Arid): Efficiency of genotyping by sequencing in inferring genomic relatedness and molecular insights into fat tail selection in Tunisian sheep

Arid

DOI	10.1111/age.13296
	Efficiency of genotyping by sequencing in inferring genomic relatedness and molecular insights into fat tail selection in Tunisian sheep
	Bedhiaf-Romdhani, Sonia; Baazaoui, Imen; Dodds, Ken G.; Brauning, Rudiger; Anderson, Rayna M.; Van Stijn, Tracey C.; McCulloch, Alan F.; McEwan, John Colin
通讯作者	Bedhiaf-Romdhani, S
来源期刊	ANIMAL GENETICS
ISSN	0268-9146
EISSN	1365-2052
出版年	2023
卷号	54 期号:3 页码:389-397
英文摘要	In developing countries, the use of simple and cost-efficient molecular technology is crucial for genetic characterization of local animal resources and better development of conservation strategies. The genotyping by sequencing (GBS) technique, also called restriction enzyme- reduced representational sequencing, is an efficient, cost-effective method for simultaneous discovery and genotyping of many markers. In the present study, we applied a two-enzyme GBS protocol (PstI/MspI) to discover and genotype SNP markers among 197 Tunisian sheep samples. A total of 100 333 bi-allelic SNPs were discovered and genotyped with an SNP call rate of 0.69 and mean sample depth 3.33. The genomic relatedness between 183 samples grouped the samples perfectly to their populations and pointed out a high genetic relatedness of inbred subpopulation reflecting the current adopted reproductive strategies. The genome-wide association study contrasting fat vs. thin-tailed breeds detected 41 significant variants including a peak positioned on OAR20. We identified FOXC1, GMDS, VEGFA, OXCT1, VRTN and BMP2 as the most promising for sheep tail-type trait. The GBS data have been useful to assess the population structure and improve our understanding of the genomic architecture of distinctive characteristics shaped by selection pressure in local sheep breeds. This study successfully investigates a cost-efficient method to discover genotypes, assign populations and understand insights into sheep adaptation to arid area. GBS could be of potential utility in livestock species in developing/emerging countries.
英文关键词	admixture GBS genomic relatedness GWAS tail fatness
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000925042000001
WOS类目	Agriculture, Dairy & Animal Science ; Genetics & Heredity
WOS研究方向	Agriculture ; Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/395300
推荐引用方式 GB/T 7714	Bedhiaf-Romdhani, Sonia,Baazaoui, Imen,Dodds, Ken G.,et al. Efficiency of genotyping by sequencing in inferring genomic relatedness and molecular insights into fat tail selection in Tunisian sheep[J],2023,54(3):389-397.
APA	Bedhiaf-Romdhani, Sonia.,Baazaoui, Imen.,Dodds, Ken G..,Brauning, Rudiger.,Anderson, Rayna M..,...&McEwan, John Colin.(2023).Efficiency of genotyping by sequencing in inferring genomic relatedness and molecular insights into fat tail selection in Tunisian sheep.ANIMAL GENETICS,54(3),389-397.
MLA	Bedhiaf-Romdhani, Sonia,et al."Efficiency of genotyping by sequencing in inferring genomic relatedness and molecular insights into fat tail selection in Tunisian sheep".ANIMAL GENETICS 54.3(2023):389-397.