干旱区生态环境知识资源中心(Arid): Changes of Soil Nitrogen Fractions and nirS-Type Denitrifier Microbial Community in Response to N Fertilizer in the Semi-Arid Area of Northeast China

Arid

DOI	10.3390/agronomy13092212
	Changes of Soil Nitrogen Fractions and nirS-Type Denitrifier Microbial Community in Response to N Fertilizer in the Semi-Arid Area of Northeast China
	Li, Yingjie; Wang, Meng; Li, Qian; Zhang, Lei; Qin, Yubo; Sun, Bo; Liu, Hang
通讯作者	Liu, H
来源期刊	AGRONOMY-BASEL
EISSN	2073-4395
出版年	2023
卷号	13 期号:9
英文摘要	The denitrification process is one of the important processes in the soil nitrogen (N) cycle, and is closely related to the loss of soil N fertilizer. Five treatments were included in this study: N0 (control, no N application); N90 (N application rate 90 kg ha(-1)); N150 (N application rate 150 kg ha(-1)); N210 (N application rate 210 kg ha(-1)); and N270 (N application rate 270 kg ha(-1)), to study the effect of different N application rates on the soil nirS-type denitrifying bacterial community structure, the influence of key enzyme activities during the denitrification process, and the main environmental factors affecting the variation of the denitrifying bacterial community in maize field soil under the mulched fertigation system in the semi-arid region of Northeast China. The results showed that increasing N fertilizer application significantly increased the contents of soil inorganic N and acid-hydrolyzable organic N, but significantly decreased pH. N fertilizer significantly increased nitrate reductase (NAR) activity and nitrite reductase (NIR) activity. Excessive application of N fertilizer significantly increased the nirS gene copy numbers, and, at the same time, significantly decreased the diversity of nirS-type soil denitrifying bacteria. Proteobacteria was the dominant denitrifying phylum in all treatments, and N application promoted the growth of Bradyrhizobium belonging to this phylum. The application of N fertilizer significantly changed the community structure of nirS denitrifying bacteria, and the NO3--N content was the most important factor for this observation. Soil organic matter (SOM) and non-hydrolyzable N (NHN) indirectly affected the denitrifying bacterial community structure through NAR activity and NIR activity, while soil total N (TN) and nitrate N (NO3--N) indirectly affected yield through denitrifying bacterial community structure. Although increasing N fertilizer was beneficial in increasing soil nutrients, the community structure of nirS-type denitrifying bacteria changed significantly. This is attributed to the increase in soil NO3--N accumulation caused by a large amount of N application. The results of this research provide an important scientific basis for further research on the response mechanism of farmland soil denitrifying microorganisms to different N fertilizer managements under the mulched fertigation system in the semi-arid region of Northeast China.
英文关键词	nitrogen denitrifying bacteria acid-hydrolyzable organic N reductase activity Illumina MiSeq sequencing
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:001074975500001
WOS关键词	LONG-TERM FERTILIZATION ; ORGANIC NITROGEN ; BACTERIA ; MANURE ; NITRIFICATION ; DIVERSITY ; SEQUENCES ; ABUNDANCE ; SYSTEM ; GENES
WOS类目	Agronomy ; Plant Sciences
WOS研究方向	Agriculture ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/395235
推荐引用方式 GB/T 7714	Li, Yingjie,Wang, Meng,Li, Qian,et al. Changes of Soil Nitrogen Fractions and nirS-Type Denitrifier Microbial Community in Response to N Fertilizer in the Semi-Arid Area of Northeast China[J],2023,13(9).
APA	Li, Yingjie.,Wang, Meng.,Li, Qian.,Zhang, Lei.,Qin, Yubo.,...&Liu, Hang.(2023).Changes of Soil Nitrogen Fractions and nirS-Type Denitrifier Microbial Community in Response to N Fertilizer in the Semi-Arid Area of Northeast China.AGRONOMY-BASEL,13(9).
MLA	Li, Yingjie,et al."Changes of Soil Nitrogen Fractions and nirS-Type Denitrifier Microbial Community in Response to N Fertilizer in the Semi-Arid Area of Northeast China".AGRONOMY-BASEL 13.9(2023).