干旱区生态环境知识资源中心(Arid): Ethylene Regulates Combined Drought and Low Nitrogen Tolerance in Wheat: Proteomic Analysis

Arid

DOI	10.3390/agronomy13071950
	Ethylene Regulates Combined Drought and Low Nitrogen Tolerance in Wheat: Proteomic Analysis
	Yan, Jiji; Wang, Daoping; Kang, Shuyu; He, Zhang; Li, Xin; Tang, Wensi; Chen, Kai; Pan, Yinghong; Zhou, Yongbin; Xu, Zhaoshi; Chen, Jun; Ma, Youzhi; Chen, Ming; Pang, Chunhua
通讯作者	Pang, CH
来源期刊	AGRONOMY-BASEL
EISSN	2073-4395
出版年	2023
卷号	13 期号:7
英文摘要	Wheat is a staple crop in China's arid and semi-arid regions. Drought and low nitrogen (LN) are two major constraints to wheat growth and production. However, the molecular mechanism underlying wheat response to both drought and LN stress remains unknown. Accordingly, we conducted a proteomic study on the roots of two wheat varieties, Chang6878 (drought tolerant) and Shi4185 (drought sensitive) and compared the differences between drought and combined drought and LN stress treatments. In total, 5143 proteins were identified, of which 163 differentially abundant proteins (DAPs) were uniquely upregulated under drought and LN stress in Chang6878. Enrichment analysis showed that DAPs were mainly involved in mitogen-activated protein kinase signaling, phenylpropanoid biosynthesis, glutathione metabolism, ethylene biosynthesis, ethylene signal transduction, and oxidation-reduction reactions. These DAPs were verified via parallel reaction monitoring and quantitative real-time polymerase chain reaction. Chang6878 was treated with the ethylene synthesis precursor 1-aminocyclopropanecarboxylic acid, and its resistance to drought and LN stress improved. After treatment with the ethylene synthesis inhibitor silver nitrate and ethylene signal transduction inhibitor 1-methylcyclopropene, drought and LN stress resistance reduced. These results provide novel insights into the tolerance mechanisms of Chang6878 to drought and LN stress by altering ethylene synthesis and signal transduction. This study provides a reference for breeding drought- and low-nitrogen-tolerant wheat germplasm resources and a theoretical basis for maintaining food security in arid, barren areas.
英文关键词	combined drought and low nitrogen stress ethylene synthesis ethylene signal transduction proteomic analysis wheat
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:001034744500001
WOS关键词	STRESS ; WATER ; GROWTH ; YIELD
WOS类目	Agronomy ; Plant Sciences
WOS研究方向	Agriculture ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/395216
推荐引用方式 GB/T 7714	Yan, Jiji,Wang, Daoping,Kang, Shuyu,et al. Ethylene Regulates Combined Drought and Low Nitrogen Tolerance in Wheat: Proteomic Analysis[J],2023,13(7).
APA	Yan, Jiji.,Wang, Daoping.,Kang, Shuyu.,He, Zhang.,Li, Xin.,...&Pang, Chunhua.(2023).Ethylene Regulates Combined Drought and Low Nitrogen Tolerance in Wheat: Proteomic Analysis.AGRONOMY-BASEL,13(7).
MLA	Yan, Jiji,et al."Ethylene Regulates Combined Drought and Low Nitrogen Tolerance in Wheat: Proteomic Analysis".AGRONOMY-BASEL 13.7(2023).