干旱区生态环境知识资源中心(Arid): Degradation of endocrine-disrupting chemicals in wastewater by new thermophilic fungal isolates and their laccases

Arid

DOI	10.1007/s13205-022-03439-1
	Degradation of endocrine-disrupting chemicals in wastewater by new thermophilic fungal isolates and their laccases
	Daassi, Dalel; Alharbi, Shuruq Rahim
通讯作者	Daâssi, D
来源期刊	3 BIOTECH
ISSN	2190-572X
EISSN	2190-5738
出版年	2023
卷号	13 期号:1
英文摘要	Thermophilic fungi are known to develop different metabolic and catabolic activities that enable them to function at elevated temperatures. Screening heat-resistant fungi, as promising resources for enzymatic activities, are still recommended. A total of eleven wood-decay thermophilic fungal strains were isolated from decaying organic materials (DOM) collected from arid areas of Khulais (Saudi Arabia). Six of these isolates are laccase-producing thermophilic strains growing at 50 degrees C. Among Laccase positive (Lac+) isolates, Chaetomium brasiliense (G3), Canariomyces notabilis (KW1), and Paecilomyces formo-sus (KW3) were exploited to treat single selected endocrine-disrupting chemicals (EDCs) that belonged to different classes (synthetic steroid hormone: 17 alpha-ethinyl estradiol (EE2), and alkylphenols:4-tert-butylphenol (4-t-BP)). Chaetomium sp. was selected due to its potentialities against target EDCs, and then, their laccases were extracted and exploited for the biocatalytic degradation of treated municipal sewage wastewaters (TMWW) mixed with 4-t-BP and EE2. The results show that within 2 h of catalyzing at 50 degrees C, laccase could degrade 60 +/- 4.8% of 4-t-BP; however, it oxidized EE2 less efficiently, reaching 35 +/- 4.1%. The influence of some redox mediators on the laccase oxidation system was investigated. The 1-hydroxybenzotria-zole (HBT) and syringaldehyde led to the highest transformation rates of EE2 (approximately 80 +/- 2.4%). Near-total removal (90 +/- 7.2%) of 4-t-BP was achieved with TEMPO in 2 h. With the metabolites identified through gas chromatography-tandem mass spectrometry (GC-MS), metabolic pathways of degradation were suggested. The results highlight the potential of Chaetomium sp. strains in the conversion of micropollutants.
英文关键词	Thermophilic Chaetomium sp Laccase Redox mediators Metabolic pathway 17 alpha-ethinylestradiol 4-tert-butylphenol
类型	Article
语种	英语
开放获取类型	Green Published
收录类别	SCI-E
WOS记录号	WOS:000925855900001
WOS关键词	BISPHENOL-A ; OXIDATION ; REMOVAL ; 17-BETA-ESTRADIOL ; BIODEGRADATION ; IDENTIFICATION ; SYSTEMS ; FATE ; EDCS
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/394929
推荐引用方式 GB/T 7714	Daassi, Dalel,Alharbi, Shuruq Rahim. Degradation of endocrine-disrupting chemicals in wastewater by new thermophilic fungal isolates and their laccases[J],2023,13(1).
APA	Daassi, Dalel,&Alharbi, Shuruq Rahim.(2023).Degradation of endocrine-disrupting chemicals in wastewater by new thermophilic fungal isolates and their laccases.3 BIOTECH,13(1).
MLA	Daassi, Dalel,et al."Degradation of endocrine-disrupting chemicals in wastewater by new thermophilic fungal isolates and their laccases".3 BIOTECH 13.1(2023).