干旱区生态环境知识资源中心(Arid): Optimization of Urease Production Capacity of a Novel Salt-Tolerant Staphylococcus xylosus Strain through Response Surface Modeling

Arid

DOI	10.3390/su142013623
	Optimization of Urease Production Capacity of a Novel Salt-Tolerant Staphylococcus xylosus Strain through Response Surface Modeling
	He, Yunxiu; Zhao, Yuan; Zhang, Wen; Zhang, Yurong; Zou, Yi
通讯作者	Zhang, W
来源期刊	SUSTAINABILITY
EISSN	2071-1050
出版年	2022
卷号	14 期号:20
英文摘要	Encouraging advances have been made in the application of microbial mineralization towards fixing and improving desertified sandy soils. However, desert soils in arid areas exhibit high salinity that may limit urease activity and production in microbial strains, thereby affecting the solidification effects of microbial calcium binders in saline soils. In this study, a salt-tolerant microbial strain (A80) that produced urease was identified from saline soils of the Qaidam Basin. The culture conditions of the strain were optimized using single-factor tests and response surface methods to optimize urease yields and activity. The optimal composition of the A80 medium included an inoculation amount of 6.32% (V/V), a yeast extract powder concentration of 15.43 g/L, a glucose concentration of 5.20 g/L, a salinity of 3%, and an incubation temperature of 36 degrees C. Urease activity increased by 64.80% after using optimized medium. The A80 microbial calcium-cementing agent was also used to solidify saline soils, leading to an increased unconfined compressive strength of the solidified saline soil by 25.70%. Thus, the optimization method resulted in improvements in the cultivation of a salt-tolerant strain.
英文关键词	salt-tolerant microorganisms microbial calcium binder urease response surface method
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E ; SSCI
WOS记录号	WOS:000873598400001
WOS关键词	MICROBIAL CARBONATE PRECIPITATION ; ASSISTED EXTRACTION ; SOIL IMPROVEMENT ; POLYSACCHARIDES ; BIOREMEDIATION ; CEMENTATION ; REMEDIATION ; DUST
WOS类目	Green & Sustainable Science & Technology ; Environmental Sciences ; Environmental Studies
WOS研究方向	Science & Technology - Other Topics ; Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/394661
推荐引用方式 GB/T 7714	He, Yunxiu,Zhao, Yuan,Zhang, Wen,et al. Optimization of Urease Production Capacity of a Novel Salt-Tolerant Staphylococcus xylosus Strain through Response Surface Modeling[J],2022,14(20).
APA	He, Yunxiu,Zhao, Yuan,Zhang, Wen,Zhang, Yurong,&Zou, Yi.(2022).Optimization of Urease Production Capacity of a Novel Salt-Tolerant Staphylococcus xylosus Strain through Response Surface Modeling.SUSTAINABILITY,14(20).
MLA	He, Yunxiu,et al."Optimization of Urease Production Capacity of a Novel Salt-Tolerant Staphylococcus xylosus Strain through Response Surface Modeling".SUSTAINABILITY 14.20(2022).