干旱区生态环境知识资源中心(Arid): Assessing phenolic and molecular diversity of arta (Calligonum comosum L.), a wild Tunisian desert plant

Arid

DOI	10.1016/j.sajb.2022.09.044
	Assessing phenolic and molecular diversity of arta (Calligonum comosum L.), a wild Tunisian desert plant
	Gasmi, Amel; Triki, Tebra; Benabderrahim, Mohamed Ali; Nagaz, Kamel; Guasmi, Ferdaous
通讯作者	Gasmi, A
来源期刊	SOUTH AFRICAN JOURNAL OF BOTANY
ISSN	0254-6299
EISSN	1727-9321
出版年	2022
卷号	151 页码:166-174
英文摘要	The species Calligonum comosum of Polygonaceae is one of the most economically important resources of the Tunisian desert, playing an important role in the lives of the local populace. This plant has been reported to possess various chemical constituents and many medicinal effects. A great range of genetic diversity could be seen in diverse populations of this species spread of south Tunisian. To better appreciate and further refine the diversity analysis, we have opted for two molecular analysis techniques, RAPD and ISSR, and one tech-nique of biochemical analysis. The extent of genetic variability was estimated in a set of seven Calligonum genotypes collected in seven Tunisian arid regions using 7 RAPD (random amplified polymorphic DNA) and 7 ISSR (inter simple sequence repeats) markers. ISSR and RAPD markers revealed 67% and 72, 85% polymorphic bands, respectively. The relative efficiencies of these marker systems are compared using various parameters such as effective multiplexing ratio (EMR), marker index (MI), and the polymorphic information content (PIC). RAPD seems to be the most informative and efficient system. A significant correlation is observed between the Jaccard dissimilarity matrices based on the ISSR and RAPD markers (r = 0.66). HPLC/MS screened methanol extracts of the seven genotypes and the identified phenolic compounds were further quantified. Significant differences were observed among the various studied genotypes regarding their phenolic com-pound content. The overall results showed that C.comosum is an excellent source of diverse bioactive mole-cules, which could be an interesting material for industrial purposes. The biochemical markers do not correlate with the ISSR and RAPD markers. These findings are important for a better understanding of the adaptive strategy of C. comosum in south Tunisia. They will be useful for conservation managers to work out an effective strategy to protect this important species.(c) 2022 SAAB. Published by Elsevier B.V. All rights reserved.
英文关键词	Calligonum comosum Genetic diversity ISSR RAPD Phytochemical analyses
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000878091900014
WOS关键词	GENETIC DIVERSITY ; MEDICINAL-PLANT ; RAPD MARKERS ; AFLP MARKERS ; ANTIOXIDANT ; FLAVONOIDS ; EXTRACTS ; ISSR ; SSR ; ANTIBACTERIAL
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/394518
推荐引用方式 GB/T 7714	Gasmi, Amel,Triki, Tebra,Benabderrahim, Mohamed Ali,et al. Assessing phenolic and molecular diversity of arta (Calligonum comosum L.), a wild Tunisian desert plant[J],2022,151:166-174.
APA	Gasmi, Amel,Triki, Tebra,Benabderrahim, Mohamed Ali,Nagaz, Kamel,&Guasmi, Ferdaous.(2022).Assessing phenolic and molecular diversity of arta (Calligonum comosum L.), a wild Tunisian desert plant.SOUTH AFRICAN JOURNAL OF BOTANY,151,166-174.
MLA	Gasmi, Amel,et al."Assessing phenolic and molecular diversity of arta (Calligonum comosum L.), a wild Tunisian desert plant".SOUTH AFRICAN JOURNAL OF BOTANY 151(2022):166-174.