干旱区生态环境知识资源中心(Arid): Soil sample storage conditions impact extracellular enzyme activity and bacterial amplicon diversity metrics in a semi-arid ecosystem

Arid

DOI	10.1016/j.soilbio.2022.108858
	Soil sample storage conditions impact extracellular enzyme activity and bacterial amplicon diversity metrics in a semi-arid ecosystem
	Lane, Jenna M.; Delavaux, Camille S.; Van Koppen, Linsey; Lu, Peina; Cade-Menun, Barbara J.; Tremblay, Julien; Bainard, Luke D.
通讯作者	Bainard, LD
来源期刊	SOIL BIOLOGY & BIOCHEMISTRY
ISSN	0038-0717
EISSN	1879-3428
出版年	2022
卷号	175
英文摘要	The analysis of microbiological and metabolic features of soils is an important aspect of soil ecology, but the results can be heavily impacted by sample storage conditions. Inconsistencies in storage methods and length of storage across studies reduce the ability to accurately collect field-based measures and compare results between projects. In this study, we examined the effects of various storage conditions and storage time on results of subsequent bacterial 16S rRNA amplicon sequencing and on activities of extracellular enzymes, using soil samples collected from three different land use types (annual cropping system, native rangeland, and riparian forest) in a semi-arid region of the Canadian Prairies. We found that when comparing enzyme activities and bacterial communities across different land use types, storage conditions may not have a significant impact. However, storage conditions were found to be important within a single land use type. Air-drying of soil samples caused significant shifts in enzyme activity and beta-diversity from the controls. Storage at-80 degrees C was best for maintaining consistent enzyme activity and microbial alpha-and beta-diversity compared to controls (i.e., freshly collected soil) across all three land use types and storage times. The results from this study provide useful in-formation about the impact of sample storage conditions for researchers in similar climates and encourage further consideration and discussion of the impacts of sample storage, as well as the reporting of storage con-ditions used in future studies.
英文关键词	Bacterial community Enzyme activity Soil storage methods 16S rRNA sequencing
类型	Article
语种	英语
开放获取类型	hybrid, Green Published
收录类别	SCI-E
WOS记录号	WOS:000884840900008
WOS关键词	RIBOSOMAL-RNA SEQUENCES ; BETA-GLUCOSIDASE ; GENE DATABASE ; PHOSPHATASE ; ECOLOGY
WOS类目	Soil Science
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/394509
推荐引用方式 GB/T 7714	Lane, Jenna M.,Delavaux, Camille S.,Van Koppen, Linsey,et al. Soil sample storage conditions impact extracellular enzyme activity and bacterial amplicon diversity metrics in a semi-arid ecosystem[J],2022,175.
APA	Lane, Jenna M..,Delavaux, Camille S..,Van Koppen, Linsey.,Lu, Peina.,Cade-Menun, Barbara J..,...&Bainard, Luke D..(2022).Soil sample storage conditions impact extracellular enzyme activity and bacterial amplicon diversity metrics in a semi-arid ecosystem.SOIL BIOLOGY & BIOCHEMISTRY,175.
MLA	Lane, Jenna M.,et al."Soil sample storage conditions impact extracellular enzyme activity and bacterial amplicon diversity metrics in a semi-arid ecosystem".SOIL BIOLOGY & BIOCHEMISTRY 175(2022).