干旱区生态环境知识资源中心(Arid): Fine genetic structure of Brazilian white Morada Nova hair sheep breed from semi-arid region

Arid

DOI	10.1016/j.smallrumres.2022.106694
	Fine genetic structure of Brazilian white Morada Nova hair sheep breed from semi-arid region
	Nunes, Samuel Freitas; Ferreira, Josiel; Paiva, Samuel Rezende; de Faria, Danielle Assis; de Sousa, Jose Ernandes Rufino; Soares, Carlos Eduardo Alves; Silveira, Robson Mateus Freitas; Mourao, Gerson Barreto; Facanha, Debora Andrea Evangelista
通讯作者	Ferreira, J
来源期刊	SMALL RUMINANT RESEARCH
ISSN	0921-4488
EISSN	1879-0941
出版年	2022
卷号	211
英文摘要	Due to the critical population status of the Morada Nova breed, this research aimed to carry out an additional study of fine genetic structure, using thousands of SNP markers, to support the development of ex situ and in situ conservation strategies for this breed. Blood samples were collected from seven herds located in the Northeast region of Brazil. DNA was extracted and samples were genotyped using Ovine SNP50 BeadChip. Raw genotyping data were imported and quality control filters were applied. The following measures of genetic variability were used to compare levels of heterogeneity within herds: Observed heterozygosity (H-O), Expected heterozygosity (H-E), Inbreeding coefficient (F) and Number of polymorphic SNPs with MAF > 0.05 (N-SNP.) The pairwise fixation index (F-ST) and an analysis of molecular variance (AMOVA) were calculated to estimate the genetic differentiation between all pairs of herds studied. To assess the existence of significant genetic structure among the herds, a principal component analysis (PCA) was performed. The HO for populations B, E, F, and G were greater than He, and the F in all subpopulations was low (F < 0.05). Population pairs D-E (F-ST = 5.9%) and D-G (F-ST = 6.2%) showed higher values of genetic differentiation from each other, and the two closest population pairs were A-F (F-ST = 0.2%) and B-E (F-ST = 0.9%). PCA corroborates the low overall genetic divergence among the populations. The white Morada Nova subpopulations studied here have low genetic differentiation among themselves, with no differentiation by states, but have three main genetic groups. The Morada Nova breed may be a repository of alleles of economic interest for the sheep production chain. These findings may initiate other studies that will make it possible to crossbreeding between the herds by identifying the herds that are more genetically distant and increasing the genetic diversity of the population.
英文关键词	Genotyping Native herds OvineSNP50 BeadChip Ovis aries Pairwise fixation index
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000797338600001
WOS关键词	POPULATION-STRUCTURE
WOS类目	Agriculture, Dairy & Animal Science
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/394497
推荐引用方式 GB/T 7714	Nunes, Samuel Freitas,Ferreira, Josiel,Paiva, Samuel Rezende,et al. Fine genetic structure of Brazilian white Morada Nova hair sheep breed from semi-arid region[J],2022,211.
APA	Nunes, Samuel Freitas.,Ferreira, Josiel.,Paiva, Samuel Rezende.,de Faria, Danielle Assis.,de Sousa, Jose Ernandes Rufino.,...&Facanha, Debora Andrea Evangelista.(2022).Fine genetic structure of Brazilian white Morada Nova hair sheep breed from semi-arid region.SMALL RUMINANT RESEARCH,211.
MLA	Nunes, Samuel Freitas,et al."Fine genetic structure of Brazilian white Morada Nova hair sheep breed from semi-arid region".SMALL RUMINANT RESEARCH 211(2022).