干旱区生态环境知识资源中心(Arid): Simultaneous Determination of Levamisole, Mebendazole, and the Two Metabolite Residues of Mebendazole in Poultry Eggs by High-Performance Liquid Chromatography-Tandem Mass Spectrometry

Arid

DOI	10.3390/separations9040083
	Simultaneous Determination of Levamisole, Mebendazole, and the Two Metabolite Residues of Mebendazole in Poultry Eggs by High-Performance Liquid Chromatography-Tandem Mass Spectrometry
	Chen, Lan; He, Zhaoyuan; Zhang, Peiyang; Guo, Yawen; Lu, Yang; Tang, Yayun; Chen, Jinyuan; Xie, Kaizhou
通讯作者	Xie, KZ
来源期刊	SEPARATIONS
EISSN	2297-8739
出版年	2022
卷号	9 期号:4
英文摘要	The quantitative determination of levamisole (LMS), mebendazole (MBZ), and the two metabolites of MBZ, 5-hydroxymebendazole (HMBZ) and 2-amino-5-benzoylbenzimidazole (AMBZ), in poultry eggs (hen, duck, and goose) was achieved with high-performance liquid chromatography-tandem triple quadrupole mass spectrometry (HPLC-MS/MS). Samples were pretreated by liquid-liquid extraction and solid-phase extraction (LLE-SPE) to extract the target compounds, and an Oasis MCX SPE column was used for purification. Determination was performed on an Xbridge C18 column with 0.1% formic acid aqueous solution and acetonitrile as mobile phases. LMS, MBZ, HMBZ, and AMBZ were detected in a triple-quadrupole mass spectrometer with ESI in positive mode and quantified with an external standard. In blank eggs, the target analyte concentrations were within the limits of quantification (LOQs)-25 mu g/kg (LMS) and 150 mu g/kg (MBZ, HMBZ, and AMBZ)-and the matrix-matched calibration curves had good linearity (R-2 >= 0.9990). In the same concentration range, the average recoveries of the target analytes were 85.98-97.38% (n. = 6); the relative standard deviation (RSD), intraday RSD, and interday RSD ranged from 2.06 to 4.22%, 1.40 to 5.85%, and 2.34 to 6.32%, respectively. The limits of detection (LODs) ranged from 0.03 to 0.33 mu g/kg, and the LOQs ranged from 0.08 to 1.00 mu g/kg. Experimental verification showed that the HPLC-MS/MS method exhibited high specificity and sensitivity for quantitative analyses of egg samples. This study provides a rapid, efficient, and sensitive method for the simultaneous detection of LMS, MBZ, HMBZ, and AMBZ residues in foods of animal origin.
英文关键词	veterinary drug residue poultry egg validation method HPLC-MS/MS LLE-SPE
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000787072700001
WOS关键词	GAS-CHROMATOGRAPHY ; VETERINARY DRUGS ; QUANTIFICATION ; TETRATHYRIDIA ; ALBENDAZOLE ; VALIDATION ; EXTRACTION ; TISSUES
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/394492
推荐引用方式 GB/T 7714	Chen, Lan,He, Zhaoyuan,Zhang, Peiyang,et al. Simultaneous Determination of Levamisole, Mebendazole, and the Two Metabolite Residues of Mebendazole in Poultry Eggs by High-Performance Liquid Chromatography-Tandem Mass Spectrometry[J],2022,9(4).
APA	Chen, Lan.,He, Zhaoyuan.,Zhang, Peiyang.,Guo, Yawen.,Lu, Yang.,...&Xie, Kaizhou.(2022).Simultaneous Determination of Levamisole, Mebendazole, and the Two Metabolite Residues of Mebendazole in Poultry Eggs by High-Performance Liquid Chromatography-Tandem Mass Spectrometry.SEPARATIONS,9(4).
MLA	Chen, Lan,et al."Simultaneous Determination of Levamisole, Mebendazole, and the Two Metabolite Residues of Mebendazole in Poultry Eggs by High-Performance Liquid Chromatography-Tandem Mass Spectrometry".SEPARATIONS 9.4(2022).