干旱区生态环境知识资源中心(Arid): Expression profiling of TaARGOS homoeologous drought responsive genes in bread wheat

Arid

DOI	10.1038/s41598-022-07637-y
	Expression profiling of TaARGOS homoeologous drought responsive genes in bread wheat
	Ahmed, Kashif; Shabbir, Ghulam; Ahmed, Mukhtar; Noor, Sabahat; Din, Atta Mohi Ud; Qamar, Maqsood; Rehman, Nazia
通讯作者	Ahmed, K
来源期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
出版年	2022
卷号	12 期号:1
英文摘要	Drought tolerant germplasm is needed to increase crop production, since water scarcity is a critical bottleneck in crop productivity worldwide. Auxin Regulated Gene involved in Organ Size (ARGOS) is a large protein family of transcription factors that plays a vital role in organ size, plant growth, development, and abiotic stress responses in plants. Although, the ARGOS gene family has been discovered and functionalized in a variety of crop plants, but a comprehensive and systematic investigation of ARGOS genes in locally used commercial wheat cultivars is still yet to be reported. The relative expression of three highly conserved TaARGOS homoeologous genes (TaARGOS-A, TaARGOS-B, TaARGOS-D) was studied in three drought-tolerant (Pakistan-2013, NARC-2009 and NR-499) and three sensitive (Borlaug-2016, NR-514 and NR-516) wheat genotypes under osmotic stress, induced by PEG-6000 at 0 (exogenous control), 2, 4, 6, and 12 h. The normalization of target genes was done using beta-actin as endogenous control, whereas DREB3, as a marker gene was also transcribed, reinforcing the prevalence of dehydration in all stress treatments. Real-time quantitative PCR revealed that osmotic stress induced expression of the three TaARGOS transcripts in different wheat seedlings at distinct timepoints. Overall, all genes exhibited significantly higher expression in the drought-tolerant genotypes as compared to the sensitive ones. For instance, the expression profile of TaARGOS-A and TaARGOS-D showed more than threefold increase at 2 h and six to sevenfold increase after 4 h of osmotic stress. However, after 6 h of osmotic stress these genes started to downregulate, and the lowest gene expression was noticed after 12 h of osmotic stress. Among all the homoeologous genes, TaARGOS-D, in particular, had a more significant influence on controlling plant growth and drought tolerance as it showed the highest expression. Altogether, TaARGOSs are involved in seedling establishment and overall plant growth. In addition, the tolerant group of genotypes had a much greater relative fold expression than the sensitive genotypes. Ultimately, Pakistan-2013 showed the highest relative expression of the studied genes than other genotypes which shows its proficiency to mitigate osmotic stress. Therefore, it could be cultivated in arid and semi-arid regions under moisture-deficient regimes. These findings advocated the molecular mechanism and regulatory roles of TaARGOS genes in plant growth and osmotic stress tolerance in contrasting groups of wheat genotypes, accompanied by the genetic nature of identified genotypes in terms of their potential for drought tolerance.
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000764883800042
WOS关键词	ORYZA-SATIVA L. ; ARABIDOPSIS ; TOLERANCE ; RICE ; OVEREXPRESSION ; GROWTH ; ARGOS ; SALT ; QTL
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/394449
推荐引用方式 GB/T 7714	Ahmed, Kashif,Shabbir, Ghulam,Ahmed, Mukhtar,et al. Expression profiling of TaARGOS homoeologous drought responsive genes in bread wheat[J],2022,12(1).
APA	Ahmed, Kashif.,Shabbir, Ghulam.,Ahmed, Mukhtar.,Noor, Sabahat.,Din, Atta Mohi Ud.,...&Rehman, Nazia.(2022).Expression profiling of TaARGOS homoeologous drought responsive genes in bread wheat.SCIENTIFIC REPORTS,12(1).
MLA	Ahmed, Kashif,et al."Expression profiling of TaARGOS homoeologous drought responsive genes in bread wheat".SCIENTIFIC REPORTS 12.1(2022).