干旱区生态环境知识资源中心(Arid): Integrated transcriptome, small RNA and degradome analysis provide insights into the transcriptional regulatory networks underlying cold acclimation in jojoba

Arid

DOI	10.1016/j.scienta.2022.111050
	Integrated transcriptome, small RNA and degradome analysis provide insights into the transcriptional regulatory networks underlying cold acclimation in jojoba
	Zheng, Lamei; Wu, Wuyun; Gao, Yunfei; Wu, Yingxin; Xu, Yixiao; Zhang, Genfa; Gao, Fei; Zhou, Yijun
通讯作者	Gao, F
来源期刊	SCIENTIA HORTICULTURAE
ISSN	0304-4238
EISSN	1879-1018
出版年	2022
卷号	299
英文摘要	Jojoba (Simmondsia chinensis) is an emerging industrial oil crop cultivated in desert dry land. Jojoba is sensitive to cold stress, which hinders its domestication in the arid areas of temperate climate regions. Here, the effects of cold acclimation on physiological parameters and gene expression in jojoba leaves were analyzed. The tolerance to cold stress in cold-acclimated jojoba seedlings was enhanced as revealed by delayed fluorescence and photosynthetic parameter measurement. The expression levels of 7520 genes were changed significantly after cold acclimation, and these genes were related to multiple biological processes including the stability of photosynthetic machinery, the enhancement of ROS scavenging ability, and protein folding and RNA processing. A total of 144 miRNAs were identified in jojoba, including 133 conserved miRNAs and 11 species-specific miRNAs, and 106 targets with high confidence of miRNA were identified by degradome sequencing, including 18 novel targets of the conserved miRNAs. Forty-two miRNAs were differentially expressed in jojoba under cold acclimation, and these miRNAs were involved in cold acclimation by negatively regulating their targets. Integrated analysis revealed that multiple gene modules might play central roles in the transcriptional regulatory networks underlying cold acclimation in jojoba, in which the ICE1-CBF-SFR6 pathway activated stress response and plant defense, miR398-CSD1/2 and miR396-glutaredoxin regulated ROS homeostasis, and miR167-GDC1 modulated the protection of photosynthetic machinery. Our study will promote understanding of the molecular mechanism of cold acclimation and provide gene markers for the genetic breeding of jojoba for improving cold stress tolerance.
英文关键词	Simmondsia chinensis Cold acclimation Leaf Multi-omics
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000791311900007
WOS关键词	CHLOROPHYLL FLUORESCENCE ; SEQUENCING REVEALS ; HIGH-TEMPERATURE ; PLASMA-MEMBRANE ; STRESS-RESPONSE ; FACTOR ICE1 ; PLANT ; CBF ; TOLERANCE ; MICRORNAS
WOS类目	Horticulture
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/394414
推荐引用方式 GB/T 7714	Zheng, Lamei,Wu, Wuyun,Gao, Yunfei,et al. Integrated transcriptome, small RNA and degradome analysis provide insights into the transcriptional regulatory networks underlying cold acclimation in jojoba[J],2022,299.
APA	Zheng, Lamei.,Wu, Wuyun.,Gao, Yunfei.,Wu, Yingxin.,Xu, Yixiao.,...&Zhou, Yijun.(2022).Integrated transcriptome, small RNA and degradome analysis provide insights into the transcriptional regulatory networks underlying cold acclimation in jojoba.SCIENTIA HORTICULTURAE,299.
MLA	Zheng, Lamei,et al."Integrated transcriptome, small RNA and degradome analysis provide insights into the transcriptional regulatory networks underlying cold acclimation in jojoba".SCIENTIA HORTICULTURAE 299(2022).