干旱区生态环境知识资源中心(Arid): Intra-/extra-cellular antibiotic resistance responses to sewage sludge composting and salinization of long-term compost applied soils

Arid

DOI	10.1016/j.scitotenv.2022.156263
	Intra-/extra-cellular antibiotic resistance responses to sewage sludge composting and salinization of long-term compost applied soils
	Ji, Xiaonan; Pan, Xiangliang
通讯作者	Pan, XL
来源期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
EISSN	1879-1026
出版年	2022
卷号	838
英文摘要	Municipal sewage sludge, a reservoir of antibiotic resistance genes (ARGs), is usually composted as fertilizer for agricultural application especially in arid and semi-arid areas. The evolution patterns of intracellular ARGs (iARGs) and extracellular ARGs (eARGs) during composting and their responses to soil salinization after long-term compost application kept unclear previously, which were systematically studied in the current study. The variation and dissemination risk of eARGs and iARGs with the salinization of farmland soils was also evaluated. Extra/intra-cellular ARGs relative abundance varied drastically through composting process. Generally, the relative abundance of the cell-free eARGs (f-eARGs) and the cell-adsorbed eARGs (a-eARGs) were 4.62 and 3.54 folds (median) higher than that of iARGs, respectively, during the entire composting process, which held true even before the sludge composting (false discovery rate, FDR p < 0.05). There was no significant difference in relative abundance between f-eARGs and aeARGs. The relative abundance of eARGs gradually decreased with composting time but was relatively higher than iARGs. It was worth noting that iARGs rebounded in the maturation phase. However, an over ten-year application of the eARG-rich compost led to much more severe contamination of iARGs than eARGs in soil. Soil salinization caused remarkable rise of eARGs by 943.34-fold (FDR p < 0.05). The variation of ARGs during composting and soil salinization was closely related to the change of microbial community structure. In compost, the bacterial communities mainly interacting with ARGs were the Firmicutes (54 unique and 35 shared core genera); and the bacterial communities playing major roles in ARGs during soil salinization were Proteobacteria (116 unique and 53 shared core genera) and Actinobacteria (52 unique and 27 shared core genera). These findings are important for assessing the transmission risk of ARGs in compost application to farmland in arid and semi-arid areas.
英文关键词	Extracellular antibiotic resistance genes Intracellular antibiotic resistance genes Compost Soil salinization Bacterial communities
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000809751300001
WOS关键词	WASTE-WATER TREATMENT ; MOBILE GENETIC ELEMENTS ; ANTIMICROBIAL RESISTANCE ; LIVESTOCK MANURE ; OSMOTIC-STRESS ; HUMAN HEALTH ; BACTERIAL ; DNA ; DEGRADATION ; COMMUNITIES
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/394385
推荐引用方式 GB/T 7714	Ji, Xiaonan,Pan, Xiangliang. Intra-/extra-cellular antibiotic resistance responses to sewage sludge composting and salinization of long-term compost applied soils[J],2022,838.
APA	Ji, Xiaonan,&Pan, Xiangliang.(2022).Intra-/extra-cellular antibiotic resistance responses to sewage sludge composting and salinization of long-term compost applied soils.SCIENCE OF THE TOTAL ENVIRONMENT,838.
MLA	Ji, Xiaonan,et al."Intra-/extra-cellular antibiotic resistance responses to sewage sludge composting and salinization of long-term compost applied soils".SCIENCE OF THE TOTAL ENVIRONMENT 838(2022).