干旱区生态环境知识资源中心(Arid): Genetic divergence between sub-accessions of the melon using molecular markers

Arid

DOI	10.5935/1806-6690.20220027
	Genetic divergence between sub-accessions of the melon using molecular markers
	de Amorim, Clisneide Coelho; da Silva, Francisco Davi; do Nascimento Bordallo, Patricia; Souza de Aragao, Fernando Antonio; de Queiroz, Manoel Abilio
通讯作者	de Amorim, CC
来源期刊	REVISTA CIENCIA AGRONOMICA
ISSN	0045-6888
EISSN	1806-6690
出版年	2022
卷号	53
英文摘要	The melon (Cucumis melo L.) is one of the most important horticultural crops in the northeast of Brazil. The characterisation of germplasm is an efficient process for managing plant genetic resources, as it contributes to better conservation and improved use in breeding programs. In the present study, the genetic divergence between sub-accessions of the melon taken from traditional agriculture in the semi-arid region of Brazil was evaluated using ISSR and RAPD markers, verifying the level of agreement with the botanical classification. Twenty-six sub-accessions preserved in the Active Germplasm Bank of Cucurbitaceae for the northeast of Brazil, located at Embrapa Semiarido in Petrolina, Pernambuco, were analysed. The binary matrix of the molecular data was used to obtain the genetic dissimilarities using the complement of the Jaccard index. The genetic dissimilarities were represented in dendrograms generated using the UPGMA method. Twenty-eight ISSR primers and 26 RAPD primers were polymorphic. A total of 686 fragments were obtained, of which 451 were polymorphic. The mean polymorphism of the RAPD markers was greater than that of the ISSR markers. The number of markers was considered sufficient to accurately determine the genetic diversity. The ISSR dendrogram allowed the formation of three groups, the RAPD dendrogram, four groups, and the joint dendrogram, three groups. The study demonstrated the genetic variability between the sub-accessions, and the strong association between the characterisation made by the molecular markers and the taxonomic classification of the genotypes under study.
英文关键词	Cucumis melo Diversity Molecular characterisation Cluster analysis
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000774291400001
WOS关键词	DIVERSITY ; ISSR ; RAPD
WOS类目	Agriculture, Multidisciplinary
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/394307
推荐引用方式 GB/T 7714	de Amorim, Clisneide Coelho,da Silva, Francisco Davi,do Nascimento Bordallo, Patricia,et al. Genetic divergence between sub-accessions of the melon using molecular markers[J],2022,53.
APA	de Amorim, Clisneide Coelho,da Silva, Francisco Davi,do Nascimento Bordallo, Patricia,Souza de Aragao, Fernando Antonio,&de Queiroz, Manoel Abilio.(2022).Genetic divergence between sub-accessions of the melon using molecular markers.REVISTA CIENCIA AGRONOMICA,53.
MLA	de Amorim, Clisneide Coelho,et al."Genetic divergence between sub-accessions of the melon using molecular markers".REVISTA CIENCIA AGRONOMICA 53(2022).