干旱区生态环境知识资源中心(Arid): Time-course transcriptome and WGCNA analysis revealed the drought response mechanism of two sunflower inbred lines

Arid

DOI	10.1371/journal.pone.0265447
	Time-course transcriptome and WGCNA analysis revealed the drought response mechanism of two sunflower inbred lines
	Wu, Yang; Wang, Yaru; Shi, Huimin; Hu, Haibo; Yi, Liuxi; Hou, Jianhua
通讯作者	Yi, LX ; Hou, JH
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2022
卷号	17 期号:4
英文摘要	Drought is one of the most serious abiotic stress factors limiting crop yields. Although sunflower is considered a moderate drought-tolerant plant, drought stress still has a negative impact on sunflower yield as cultivation expands into arid regions. The extent of drought stress is varieties and time-dependent, however, the molecular response mechanisms of drought tolerance in sunflower with different varieties are still unclear. Here, we performed comparative physiological and transcriptome analyses on two sunflower inbred lines with different drought tolerance at the seedling stage. The analysis of nine physiological and biochemical indicators showed that the leaf surface area, leaf relative water content, and cell membrane integrity of drought tolerance inbred line were higher than those of drought-sensitive inbred line under drought stress, indicating that DT had stronger drought resistance. Transcriptome analyses identified 24,234 differentially expressed genes (DEGs). Gene ontology (GO) analysis showed the up-regulated genes were mainly enriched in gibberellin metabolism and rRNA processing, while the down-regulated genes were mainly enriched in cell-wall, photosynthesis, and terpene metabolism. Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis showed genes related to GABAergic synapse, ribosome biogenesis were up-regulated, while genes related with amino sugar and nucleotide sugar metabolism, starch and sucrose metabolism, photosynthesis were down-regulated. Mapman analysis revealed differences in plant hormone-signaling genes over time and between samples. A total of 1,311 unique putative transcription factors (TFs) were identified from all DEGs by iTAK, among which the high abundance of transcription factor families include bHLH, AP2/ERF, MYB, C2H2, etc. Weighted gene co-expression network analysis (WGCNA) revealed a total of 2,251 genes belonging to two modules(blue 4, lightslateblue), respectively, which were significantly associated with six traits. GO and KEGG enrichment analysis of these genes was performed, followed by visualization with Cytoscape software, and the top 20 Hub genes were screened using the CytoHubba plugin.
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000804748600020
WOS关键词	HELIANTHUS-ANNUUS L. ; STRESS TOLERANCE ; GENE-EXPRESSION ; ABSCISIC-ACID ; WATER-STRESS ; GENOME-WIDE ; ANTIOXIDATIVE ENZYMES ; SIGNALING PATHWAYS ; METHYL JASMONATE ; MAIZE SEEDLINGS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/394027
推荐引用方式 GB/T 7714	Wu, Yang,Wang, Yaru,Shi, Huimin,et al. Time-course transcriptome and WGCNA analysis revealed the drought response mechanism of two sunflower inbred lines[J],2022,17(4).
APA	Wu, Yang,Wang, Yaru,Shi, Huimin,Hu, Haibo,Yi, Liuxi,&Hou, Jianhua.(2022).Time-course transcriptome and WGCNA analysis revealed the drought response mechanism of two sunflower inbred lines.PLOS ONE,17(4).
MLA	Wu, Yang,et al."Time-course transcriptome and WGCNA analysis revealed the drought response mechanism of two sunflower inbred lines".PLOS ONE 17.4(2022).