干旱区生态环境知识资源中心(Arid): Early Growth Performance of In Vitro Raised Melia volkensii Gurke Plantlets in Response to Beneficial Microorganisms under Semi-Arid Conditions

Arid

DOI	10.3390/plants11101300
	Early Growth Performance of In Vitro Raised Melia volkensii Gurke Plantlets in Response to Beneficial Microorganisms under Semi-Arid Conditions
	Dushimimana, Constantin; Sakha, Michael Ajanja; Korir, Mercy Jebiwott; Jefwa, Joyce Mnyazi; Vandenabeele, Jan; Magomere, Titus; Mutitu, Eunice Wanjiru; Mulatya, Jackson; Olubayo, Florence; Smagghe, Guy; Werbrouck, Stefaan P. O.
通讯作者	Dushimimana, C ; Werbrouck, SPO
来源期刊	PLANTS-BASEL
ISSN	2223-7747
出版年	2022
卷号	11 期号:10
英文摘要	Before in vitro propagated Melia volkensii plants can be used for mass planting, the transition phase to in vivo conditions needs to be better controlled because too many plants are lost during acclimatization and in the field. Two experiments were set up to evaluate the effects of biological agents on the establishment of M. volkensii in vitro plantlets. The biological agents consisted of Trichotech (R) , Bio-cure B (R), Rhizatech (R) , Bacillus subtilis, a Trichoderma isolate and self-isolated native arbuscular mycorrhizal fungi (AMF). Regarding the latter, in soil from the nursery, the number of AMF spores increased from six spores to 400 per 100 g of soil using a trap culture, in which thirteen AMF morphotypes were identified and root colonization assessed through observation of hyphae, vesicles, coils and appressoria. The first experiment was set up in the greenhouse to investigate the efficacy of the biological agents on the hardening off. In the second, a field experiment was set up to study their effect on the early establishment of the plantlets in the field compared to seedlings. All biological agents significantly (p <= 0.05) improved in vitro plant survival and growth compared to the control. The highest plant height and number of leaves per plant were recorded in plants treated with Rhizatech (R) , Native AMF, Bio-cure B (R) and Trichoderma isolate. The treatments with Rhizatech (R) , Bio-cure B (R) and native mycorrhiza recorded a significantly wider stem. The root diameter of the plants treated with Rhizatech (R) and Bio-cure B (R) was the largest, but the plants inoculated with the native AMF had the longest roots. Moreover, the inoculated plants generally developed multiple secondary roots. After two months, AMF had clearly colonized the acclimatized plantlets. In the field experiment, the biologicals made no difference in survival rate but did produce a significantly larger leaf area after two months, with the largest leaves recorded with Rhizatech (R) , native AMF and Trichotech (R) . They also increased the quality index of the plants from 0.21 to 0.52. The performance of in vitro grown M. volkensii plants six months after planting in semi-arid conditions in Kiambere was better than that of seedlings. Inoculation of plants increased plant height and diameter. Thus, inoculation of biological agents is an efficient approach for improving the early growth of in vitro propagated M. volkensii plants.
英文关键词	Melia volkensii biological agents micropropagation acclimatization field arid and semi-arid areas
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000802509200001
WOS关键词	ARBUSCULAR MYCORRHIZAL FUNGI ; PSEUDOMONAS-FLUORESCENS ; MICROPROPAGATED PLANTS ; TISSUE-CULTURE ; ACCLIMATIZATION ; REGENERATION ; SEEDLINGS ; ROOTS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393986
推荐引用方式 GB/T 7714	Dushimimana, Constantin,Sakha, Michael Ajanja,Korir, Mercy Jebiwott,et al. Early Growth Performance of In Vitro Raised Melia volkensii Gurke Plantlets in Response to Beneficial Microorganisms under Semi-Arid Conditions[J],2022,11(10).
APA	Dushimimana, Constantin.,Sakha, Michael Ajanja.,Korir, Mercy Jebiwott.,Jefwa, Joyce Mnyazi.,Vandenabeele, Jan.,...&Werbrouck, Stefaan P. O..(2022).Early Growth Performance of In Vitro Raised Melia volkensii Gurke Plantlets in Response to Beneficial Microorganisms under Semi-Arid Conditions.PLANTS-BASEL,11(10).
MLA	Dushimimana, Constantin,et al."Early Growth Performance of In Vitro Raised Melia volkensii Gurke Plantlets in Response to Beneficial Microorganisms under Semi-Arid Conditions".PLANTS-BASEL 11.10(2022).