干旱区生态环境知识资源中心(Arid): Transcriptomic and metabolomics-based analysis of key biological pathways reveals the role of lipid metabolism in response to salt stress in the root system of Brassica napus

Arid

DOI	10.1007/s10725-021-00788-4
	Transcriptomic and metabolomics-based analysis of key biological pathways reveals the role of lipid metabolism in response to salt stress in the root system of Brassica napus
	Wang, Weichao; Pang, Jiayin; Zhang, Fenghua; Sun, Lupeng; Yang, Lei; Siddique, Kadambot H. M.
通讯作者	Zhang, FH
来源期刊	PLANT GROWTH REGULATION
ISSN	0167-6903
EISSN	1573-5087
出版年	2022
卷号	97 期号:1 页码:127-141
英文摘要	Soil salinity is a major constraint affecting crop growth and productivity, and limiting sustainable agricultural development in arid zones. Understanding the molecular mechanisms underlying the adaptation of canola to salt stress is important to improve salt tolerance and promote its cultivation in saline soils. To elucidate the metabolic and transcriptional regulatory mechanisms in canola under salt stress, the seedling roots of the control (no salt treatment) and roots of canola seedlings subjected to 72 h of 200 mM NaCl stress (hydroponics) were collected for metabolomic analysis, supplemented with RNA-Seq analysis and quantitative real-time PCR (qRT-PCR) validation. Metabolomic analysis showed that compared with the control, the metabolites of lipids accumulated more under NaCl stress, including unsaturated fatty acids (linoleic acid, dihomo-gamma-linolenic acid, oleic acid, nervonic acid, alpha-linolenic acid), glycerophospholipids (1-palmitoyl-sn-glycero-3-phosphocholine, 1-oleoyl-sn-glycero-3-phosphocholine, 1-stearoyl-2-hydroxy-sn-glycero-3-phosphocholine) lecithin (phosphorylcholine), sphingolipids (N-palmitoylsphingosine) and steroids and steroid derivatives (25-hydroxyvitamin D3); while the metabolism of most amino acids (such as l-valine, l-threonine, l-isoleucine, l-glutamate, l-phenylalanine) and carbohydrates (such as d-fructose, cellobiose, sucrose, d-mannose) were lower. Both transcriptomic and metabolomic pathway analysis indicated that lipid metabolism was an important metabolic pathway in canola roots under NaCl stress. In summary, canola seedling roots could respond to NaCl stress through lipid metabolism genes and metabolites, which improved our knowledge in molecular mechanisms encoding NaCl tolerance in canola.
英文关键词	Neutral salts Hydroponics Metabolite profiling KEGG pathway Lipid metabolism
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000768692100001
WOS关键词	SALINITY STRESS ; PHYSIOLOGICAL-RESPONSES ; K+/NA+ HOMEOSTASIS ; PHOSPHATIDIC-ACID ; TOLERANCE ; DROUGHT ; GROWTH ; PLANTS ; SOIL ; MECHANISMS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393968
推荐引用方式 GB/T 7714	Wang, Weichao,Pang, Jiayin,Zhang, Fenghua,et al. Transcriptomic and metabolomics-based analysis of key biological pathways reveals the role of lipid metabolism in response to salt stress in the root system of Brassica napus[J],2022,97(1):127-141.
APA	Wang, Weichao,Pang, Jiayin,Zhang, Fenghua,Sun, Lupeng,Yang, Lei,&Siddique, Kadambot H. M..(2022).Transcriptomic and metabolomics-based analysis of key biological pathways reveals the role of lipid metabolism in response to salt stress in the root system of Brassica napus.PLANT GROWTH REGULATION,97(1),127-141.
MLA	Wang, Weichao,et al."Transcriptomic and metabolomics-based analysis of key biological pathways reveals the role of lipid metabolism in response to salt stress in the root system of Brassica napus".PLANT GROWTH REGULATION 97.1(2022):127-141.