干旱区生态环境知识资源中心(Arid): Genome survey sequencing of wild cotton (Gossypium robinsonii) reveals insights into proteomic responses of pollen to extreme heat

Arid

DOI	10.1111/pce.14268
	Genome survey sequencing of wild cotton (Gossypium robinsonii) reveals insights into proteomic responses of pollen to extreme heat
	Masoomi-Aladizgeh, Farhad; Kamath, Karthik Shantharam; Haynes, Paul A.; Atwell, Brian J.
通讯作者	Atwell, BJ
来源期刊	PLANT CELL AND ENVIRONMENT
ISSN	0140-7791
EISSN	1365-3040
出版年	2022
卷号	45 期号:4 页码:1242-1256
英文摘要	Heat stress specifically affects fertility by impairing pollen viability but cotton wild relatives successfully reproduce in hot savannas where they evolved. An Australian arid-zone cotton (Gossypium robinsonii) was exposed to heat events during pollen development then mature pollen was subjected to deep proteomic analysis using 57 023 predicted genes from a genomic database we assembled for the same species. Three stages of pollen development, including tetrads (TEs), uninucleate microspores (UNs) and binucleate microspores (BNs) were exposed to 36 degrees C or 40 degrees C for 5 days and the resulting mature pollen was collected at anthesis (p-TE, p-UN and p-BN, respectively). Using the sequential windowed acquisition of all theoretical mass spectra proteomic analysis, 2704 proteins were identified and quantified across all pollen samples analysed. Proteins predominantly decreased in abundance at all stages in response to heat, particularly after exposure of TEs to 40 degrees C. Functional enrichment analyses demonstrated that extreme heat increased the abundance of proteins that contributed to increased messenger RNA splicing via spliceosome, initiation of cytoplasmic translation and protein refolding in p-TE40. However, other functional categories that contributed to intercellular transport were inhibited in p-TE40, linked potentially to Rab proteins. We ascribe the resilience of reproductive processes in G. robinsonii at temperatures up to 40 degrees C, relative to commercial cotton, to a targeted reduction in protein transport.
英文关键词	crop wild relatives heat stress metabolic down-regulation pollen development SWATH-MS tetrads thermotolerance
类型	Article
语种	英语
开放获取类型	Green Submitted, Green Published
收录类别	SCI-E
WOS记录号	WOS:000765149500001
WOS关键词	ENVIRONMENTAL-STRESS ; MALE-STERILITY ; TEMPERATURE ; PLANTS ; ADAPTATION ; CELL
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393962
推荐引用方式 GB/T 7714	Masoomi-Aladizgeh, Farhad,Kamath, Karthik Shantharam,Haynes, Paul A.,et al. Genome survey sequencing of wild cotton (Gossypium robinsonii) reveals insights into proteomic responses of pollen to extreme heat[J],2022,45(4):1242-1256.
APA	Masoomi-Aladizgeh, Farhad,Kamath, Karthik Shantharam,Haynes, Paul A.,&Atwell, Brian J..(2022).Genome survey sequencing of wild cotton (Gossypium robinsonii) reveals insights into proteomic responses of pollen to extreme heat.PLANT CELL AND ENVIRONMENT,45(4),1242-1256.
MLA	Masoomi-Aladizgeh, Farhad,et al."Genome survey sequencing of wild cotton (Gossypium robinsonii) reveals insights into proteomic responses of pollen to extreme heat".PLANT CELL AND ENVIRONMENT 45.4(2022):1242-1256.