干旱区生态环境知识资源中心(Arid): Function-Based Rhizosphere Assembly along a Gradient of Desiccation in the Former Aral Sea

Arid

DOI	10.1128/msystems.00739-22
	Function-Based Rhizosphere Assembly along a Gradient of Desiccation in the Former Aral Sea
	Wicaksono, Wisnu Adi; Egamberdieva, Dilfuza; Berg, Christian; Mora, Maximilian; Kusstatscher, Peter; Cernava, Tomislav; Berg, Gabriele
通讯作者	Wicaksono, WA ; Cernava, T ; Berg, G
来源期刊	MSYSTEMS
ISSN	2379-5077
出版年	2022
卷号	7 期号:6
英文摘要	The desiccation of the Aral Sea represents one of the largest human-made environmental regional disasters. The salt- and toxin-enriched dried-out basin provides a natural laboratory for studying ecosystem functioning and rhizosphere assembly under extreme anthropogenic conditions. Here, we investigated the prokaryotic rhizosphere communities of the native pioneer plant Suaeda acuminata (C.A.Mey.) Moq. in comparison to bulk soil across a gradient of desiccation (5, 10, and 40 years) by metagenome and amplicon sequencing combined with quantitative PCR (qPCR) analyses. The rhizosphere effect was evident due to significantly higher bacterial abundances but less diversity in the rhizosphere compared to bulk soil. Interestingly, in the highest salinity (5 years of desiccation), rhizosphere functions were mainly provided by archaeal communities. Along the desiccation gradient, we observed a significant change in the rhizosphere microbiota, which was reflected by (i) a decreasing archaeon-bacterium ratio, (ii) replacement of halophilic archaea by specific plant-associated bacteria, i.e., Alphaproteobacteria and Actinobacteria, and (iii) an adaptation of specific, potentially plant-beneficial biosynthetic pathways. In general, both bacteria and archaea were found to be involved in carbon cycling and fixation, as well as methane and nitrogen metabolism. Analysis of metagenome-assembled genomes (MAGs) showed specific signatures for production of osmoprotectants, assimilatory nitrate reduction, and transport system induction. Our results provide evidence that rhizosphere assembly by cofiltering specific taxa with distinct traits is a mechanism which allows plants to thrive under extreme conditions. Overall, our findings highlight a function-based rhizosphere assembly, the importance of plant-microbe interactions in salinated soils, and their exploitation potential for ecosystem restoration approaches.IMPORTANCE The desertification of the Aral Sea basin in Uzbekistan and Kazakhstan represents one of the most serious anthropogenic environmental disasters of the last century. Since the 1960s, the world's fourth-largest inland body of water has been constantly shrinking, which has resulted in an extreme increase of salinity accompanied by accumulation of many hazardous and carcinogenic substances, as well as heavy metals, in the dried-out basin. Here, we investigated bacterial and archaeal communities in the rhizosphere of pioneer plants by combining classic molecular methods with amplicon sequencing as well as metagenomics for functional insights. By implementing a desiccation gradient, we observed (i) remarkable differences in the archaeon-bacterium ratio of plant rhizosphere samples, (ii) replacement of archaeal indicator taxa during succession, and (iii) the presence of specific, potentially plant-beneficial biosynthetic pathways in archaea present during the early stages. In addition, our results provide hitherto-undescribed insights into the functional redundancy between plant-associated archaea and bacteria. The desertification of the Aral Sea basin in Uzbekistan and Kazakhstan represents one of the most serious anthropogenic environmental disasters of the last century. Since the 1960s, the world's fourth-largest inland body of water has been constantly shrinking, which has resulted in an extreme increase of salinity accompanied by accumulation of many hazardous and carcinogenic substances, as well as heavy metals, in the dried-out basin.
英文关键词	Aral Sea microbiome desiccation nutrient cycling soil microorganisms revegetation archaea bacteria metagenome
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000885575600001
WOS关键词	PLANT ; MICROBIOME ; SEQUENCE ; HALOPHYTES ; ALIGNMENT ; BACTERIA ; GENOMES ; ARCHAEA ; QUALITY ; PROTEIN
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393825
推荐引用方式 GB/T 7714	Wicaksono, Wisnu Adi,Egamberdieva, Dilfuza,Berg, Christian,et al. Function-Based Rhizosphere Assembly along a Gradient of Desiccation in the Former Aral Sea[J],2022,7(6).
APA	Wicaksono, Wisnu Adi.,Egamberdieva, Dilfuza.,Berg, Christian.,Mora, Maximilian.,Kusstatscher, Peter.,...&Berg, Gabriele.(2022).Function-Based Rhizosphere Assembly along a Gradient of Desiccation in the Former Aral Sea.MSYSTEMS,7(6).
MLA	Wicaksono, Wisnu Adi,et al."Function-Based Rhizosphere Assembly along a Gradient of Desiccation in the Former Aral Sea".MSYSTEMS 7.6(2022).