干旱区生态环境知识资源中心(Arid): Development of a SPE-LC-MS Method for the Quantitation of Palbociclib and Abemaciclib in Human Plasma

Arid

DOI	10.3390/molecules27238604
	Development of a SPE-LC-MS Method for the Quantitation of Palbociclib and Abemaciclib in Human Plasma
	Calucica, Daniela Maria; Manda, Costel-Valentin; Gaman, Amelia Maria; Raileanu, Stefan; Stanca, Liliana; Popescu, Monica Denisa Elena; Mateescu, Olivia Garofita; Bita, Andrei; Croitoru, Octavian; Neamtu, Simona-Daniela
通讯作者	Manda, CV
来源期刊	MOLECULES
EISSN	1420-3049
出版年	2022
卷号	27 期号:23
英文摘要	Palbociclib and abemaciclib are two cyclin-dependent kinases 4 and 6 used for breast cancer treatment. Levels of these medicines present a significant interindividual variability, so monitoring those concentrations might be necessary in therapy. Most of the methods presented so far in the literature use simple protein precipitation of plasma proteins as sample preparation method followed by direct injection of the supernatant into the LC instrument, preceded or not by a simple filtration step. Within that approach, the probability of injecting proteins in the chromatographic system is increased. With the purpose of obtaining a cleaner extract of the drugs, we developed and validated a simple and accurate LC-MS method for determining palbociclib and abemaciclib in human plasma. Solid phase extraction (SPE) using Oasis PRiME HLB (R) cartridges was used for plasma sample preparation. The method provided clean extracts with a recovery extraction higher than 85% for both compounds. Separation was achieved by high-performance liquid chromatography (HPLC), using a C18 (4.6 x 50 mm) column, with a gradient elution of ammonium acetate/acetic acid-acetonitrile as the mobile phase. Detection was performed by mass spectrometry (MS) in single ion recording (SIR) mode. Intra-day and inter-day precision data for both analytes were 3.8-7.2% and 3.6-7.4%, respectively. Calibration curves were both linear between 2 and 400 ng/mL with a correlation coefficient higher than 0.998. The LC-MS method can be used to quantify the drugs in human plasma in routine analysis. The method proved to be useful in determining real plasma levels in patients involved in cancer therapy. Drug concentrations were determined in a 10 min run-time, including re-equilibration of the column.
英文关键词	palbociclib abemaciclib human plasma SPE LC-MS
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000896618900001
WOS关键词	INHIBITOR
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393820
推荐引用方式 GB/T 7714	Calucica, Daniela Maria,Manda, Costel-Valentin,Gaman, Amelia Maria,et al. Development of a SPE-LC-MS Method for the Quantitation of Palbociclib and Abemaciclib in Human Plasma[J],2022,27(23).
APA	Calucica, Daniela Maria.,Manda, Costel-Valentin.,Gaman, Amelia Maria.,Raileanu, Stefan.,Stanca, Liliana.,...&Neamtu, Simona-Daniela.(2022).Development of a SPE-LC-MS Method for the Quantitation of Palbociclib and Abemaciclib in Human Plasma.MOLECULES,27(23).
MLA	Calucica, Daniela Maria,et al."Development of a SPE-LC-MS Method for the Quantitation of Palbociclib and Abemaciclib in Human Plasma".MOLECULES 27.23(2022).