干旱区生态环境知识资源中心(Arid): Cryptosporidium's burden of disease attributable to consumption of wastewater-irrigated raw vegetables

Arid

DOI	10.1016/j.mran.2022.100218
	Cryptosporidium's burden of disease attributable to consumption of wastewater-irrigated raw vegetables
	Farhadkhani, Marzieh; Nikaeen, Mahnaz; Hadi, Mahdi; Nikaein, Hossein; Alum, Absar; Abbaszadegan, Morteza
通讯作者	Nikaeen, M
来源期刊	MICROBIAL RISK ANALYSIS
ISSN	2352-3522
EISSN	2352-3530
出版年	2022
卷号	21
英文摘要	Conventional wastewater treatment plants do not effectively remove parasitic protozoa resulting in their pres-ence in wastewater effluent. Therefore, agricultural reuse of wastewater effluent could be a route of Crypto-sporidium transmission into the food chain. Samples of secondary wastewater, wastewater-irrigated soil and vegetable samples were collected from an experimental field and analyzed using real-time PCR for quantification of Cryptosporidium oocysts. Quantitative microbial risk analysis (QMRA) was performed to determine the annual disease burden from Cryptosporidium associated with consumption of wastewater-irrigated vegetables. Detection of Clostridium perfringens spores as a potential indicator for the presence of Cryptosporidium was also performed. Cryptosporidium was observed in 64% of the effluent samples ranging from 16 to 162 oocysts L-1, whereas no oocysts were detected in wastewater-irrigated soil and the vegetable samples. The mean annual disease burden for consumption of lettuce (6.6 x 10(-5) DALY per person per year (pppy)) was higher than spring onion (3.4 x 10(-6 )DALY pppy) which both exceeding the WHO guideline of 10-6 DALYs pppy. However, the disease burden was within the acceptable range considering the less stringent level of 10(-4 )DALY pppy. Clostridium spores were detected in 92% of the effluent samples with no relationship with the presence of Cryptosporidium. The results of this study suggest that agricultural reuse of treated wastewater may not be a major health concern of crypto-sporidiosis in a semi-arid region. However, because of the higher risk from lettuce, the choice of an appropriate crop would be needed to completely meet the WHO recommendations for safe reuse of wastewater. The accuracy of the QMRA model could be improved by further investigating the decay rate of oocysts in the same region.
英文关键词	Cryptosporidium Real-time PCR Wastewater reuse Health risk QMRA
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000814786500007
WOS关键词	MICROBIAL RISK-ASSESSMENT ; PARVUM OOCYSTS ; ASSESSMENT MODEL ; GIARDIA CYSTS ; DIE-OFF ; SOIL ; PCR ; CONTAMINATION ; EXPOSURE ; SURFACE
WOS类目	Environmental Sciences ; Food Science & Technology ; Microbiology
WOS研究方向	Environmental Sciences & Ecology ; Food Science & Technology ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393783
推荐引用方式 GB/T 7714	Farhadkhani, Marzieh,Nikaeen, Mahnaz,Hadi, Mahdi,et al. Cryptosporidium's burden of disease attributable to consumption of wastewater-irrigated raw vegetables[J],2022,21.
APA	Farhadkhani, Marzieh,Nikaeen, Mahnaz,Hadi, Mahdi,Nikaein, Hossein,Alum, Absar,&Abbaszadegan, Morteza.(2022).Cryptosporidium's burden of disease attributable to consumption of wastewater-irrigated raw vegetables.MICROBIAL RISK ANALYSIS,21.
MLA	Farhadkhani, Marzieh,et al."Cryptosporidium's burden of disease attributable to consumption of wastewater-irrigated raw vegetables".MICROBIAL RISK ANALYSIS 21(2022).