干旱区生态环境知识资源中心(Arid): Exploring Functional Diversity and Community Structure of Diazotrophic Endophytic Bacteria Associated with Pennisetum glaucum Growing under Field in a Semi-Arid Region

Arid

DOI	10.3390/land11070991
	Exploring Functional Diversity and Community Structure of Diazotrophic Endophytic Bacteria Associated with Pennisetum glaucum Growing under Field in a Semi-Arid Region
	Gupta, Garima; Paul, Sangeeta; Singh, Sachidanand; Pietramellara, Giacomo; Pathan, Shamina Imran; Danish, Subhan; Jabborova, Dilfuza; Datta, Rahul; Jha, Prabhat Nath
通讯作者	Gupta, G
来源期刊	LAND
EISSN	2073-445X
出版年	2022
卷号	11 期号:7
英文摘要	Diazotrophic endophytic bacteria (DEB) are the key drivers of nitrogen fixation in rainfed soil ecosystems and, hence, can influence the growth and yield of crop plants. Therefore, the present work investigated the structure and composition of the DEB community at different growth stages of field-grown pearl millet plants, employing the cultivation-dependent method. Diazotrophy of the bacterial isolates was confirmed by acetylene reduction assay and amplification of the nifH gene. ERIC-PCR-based DNA fingerprinting, followed by 16S rRNA gene analysis of isolates recovered at different time intervals, demonstrated the highest bacterial diversity during early (up to 28 DAS (Days after sowing)) and late (63 DAS onwards) stages, as compared to the vegetative growth stage (28-56 DAS). Among all species, Pseudomonas aeruginosa was the most dominant endophyte. Assuming modulation of the immune response as one of the tactics for successful colonization of P. aeruginosa PM389, we studied the expression of the profile of defense genes of wheat, used as a host plant, in response to P. aeruginosa inoculation. Most of the pathogenesis-related PR genes were induced initially (at 6 h after infection (HAI)), followed by their downregulation at 12 HAI. The trend of bacterial colonization was quantified by qPCR of 16S rRNAs. The results obtained in the present study indicated an attenuated defense response in host plants towards endophytic bacteria, which is an important feature that helps endophytes establish themselves inside the endosphere of roots.
英文关键词	ERIC nitrogen fixation pearl millet qPCR PM389 PR genes Pseudomonas aeruginosa
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SSCI
WOS记录号	WOS:000831443000001
WOS关键词	PATHOGENESIS-RELATED PROTEINS ; GROWTH-PROMOTING RHIZOBACTERIA ; PSEUDOMONAS-AERUGINOSA ; ARABIDOPSIS ROOTS ; GENE-EXPRESSION ; QUALITY-CONTROL ; PLANT ; COLONIZATION ; DEFENSE ; RHIZOSPHERE
WOS类目	Environmental Studies
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393678
推荐引用方式 GB/T 7714	Gupta, Garima,Paul, Sangeeta,Singh, Sachidanand,et al. Exploring Functional Diversity and Community Structure of Diazotrophic Endophytic Bacteria Associated with Pennisetum glaucum Growing under Field in a Semi-Arid Region[J],2022,11(7).
APA	Gupta, Garima.,Paul, Sangeeta.,Singh, Sachidanand.,Pietramellara, Giacomo.,Pathan, Shamina Imran.,...&Jha, Prabhat Nath.(2022).Exploring Functional Diversity and Community Structure of Diazotrophic Endophytic Bacteria Associated with Pennisetum glaucum Growing under Field in a Semi-Arid Region.LAND,11(7).
MLA	Gupta, Garima,et al."Exploring Functional Diversity and Community Structure of Diazotrophic Endophytic Bacteria Associated with Pennisetum glaucum Growing under Field in a Semi-Arid Region".LAND 11.7(2022).