干旱区生态环境知识资源中心(Arid): Genome-based identification of beet curly top Iran virus infecting sugar beet in Turkey and investigation of its pathogenicity by agroinfection

Arid

DOI	10.1016/j.jviromet.2021.114380
	Genome-based identification of beet curly top Iran virus infecting sugar beet in Turkey and investigation of its pathogenicity by agroinfection
	Yildirim, Kubilay; Kavas, Musa; Kaya, Riza; Secgin, Zafer; Can, Cansu; Sevgen, Ilkay; Gokcek Sarac, Cigdem; Tahan, Vahid
通讯作者	Yildirim, K
来源期刊	JOURNAL OF VIROLOGICAL METHODS
ISSN	0166-0934
EISSN	1879-0984
出版年	2022
卷号	300
英文摘要	Beet curly top disease (BCTD) is a yield-limiting viral infection of sugar beet (Beta vulgaris) throughout the arid and semi-arid regions of the world. Two virus species, belonging to two different genera of the family Geminiviridae (Curtovirus and Becurtovirus) had been described as the disease's causative agents on sugar beet. Despite the detection of the BCTD in some sugar beet fields of Turkey sixty years ago, the genome based characterization of BCTD-associated viruses have not been studied previously. In this study, 628 sugar beet plants exhibiting BCTD symptoms were collected from fourteen cities in central Anatolia, the major sugar beet production areas in Turkey. PCR assays of these samples using the respective Curtovirus and Becurtovirus genusspecific primers indicated that the Turkish sugar beet samples' viral sequences belong only to the genus Becurtovirus. The results of sequencing and phylogenetic analysis of the partial genome of the virus obtained from fourteen cities confirmed that BCTD-associated virus in Turkish sugar beet fields is beet curly top Iran virus (BCTIV-Becurtovirus) species. The whole genome of the collected viruses from fourteen cities were amplified by the rolling circle amplification (RCA) and the five most phylogenetically diverse viruses obtained from Afyon, Ankara, Adapazari, Yozgat and Aksaray were sequenced. The results of whole genome sequence analysis indicated >98 % sequence identities with that of a BCTIV variants reported from Urmia province (bordering Turkey) of Iran. A virus genome from Yozgat city had a genomic sequence identity of >97 % with those of BCTIV isolated from cowpea, tomato, pepper and sugar beet in the northern part of Iran. These results suggested that the spread of BCTIV through the region could create a significant threat to the production of sugar beet as well as other agricultural crops. A tandem dimer of a BCTIV-Turkish variant isolated from Ankara city was cloned into Agrobacterium plasmid to be used for agro-infection studies. Agroinoculation of this construct on sugar beet leaves generated severe BCTD symptoms (84 %) which were also confirmed by RCA and qPCR analysis. These results constituted the first genome based characterization of BCTIV Turkish variants and the first report of BCTIV spreading out of Iran.
英文关键词	Sugar beet Beet curly top Iran virus Turkey Becurtovirus Agroinfection
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000765937000007
WOS关键词	GEMINIVIRUS ; RESISTANCE ; PLANTS ; DIVERSITY ; PROPOSAL ; GENERA ; NUMBER
WOS类目	Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393632
推荐引用方式 GB/T 7714	Yildirim, Kubilay,Kavas, Musa,Kaya, Riza,et al. Genome-based identification of beet curly top Iran virus infecting sugar beet in Turkey and investigation of its pathogenicity by agroinfection[J],2022,300.
APA	Yildirim, Kubilay.,Kavas, Musa.,Kaya, Riza.,Secgin, Zafer.,Can, Cansu.,...&Tahan, Vahid.(2022).Genome-based identification of beet curly top Iran virus infecting sugar beet in Turkey and investigation of its pathogenicity by agroinfection.JOURNAL OF VIROLOGICAL METHODS,300.
MLA	Yildirim, Kubilay,et al."Genome-based identification of beet curly top Iran virus infecting sugar beet in Turkey and investigation of its pathogenicity by agroinfection".JOURNAL OF VIROLOGICAL METHODS 300(2022).