干旱区生态环境知识资源中心(Arid): Dynamics of heat shock proteins and heat shock factor expression during heat stress in daughter workers in pre-heat-treated (rapid heat hardening) Apis mellifera mother queens

Arid

DOI	10.1016/j.jtherbio.2022.103194
	Dynamics of heat shock proteins and heat shock factor expression during heat stress in daughter workers in pre-heat-treated (rapid heat hardening) Apis mellifera mother queens
	Al-Ghzawi, Abd Al-Majeed A.; Al-Zghoul, Mohammad Borhan; Zaitoun, Shahera; Al-Omary, Ilham Mustafa; Alahmad, Nour Alhoda
通讯作者	Al-Zghoul, MB
来源期刊	JOURNAL OF THERMAL BIOLOGY
ISSN	0306-4565
EISSN	1879-0992
出版年	2022
卷号	104
英文摘要	Honeybees have a limited capacity to control their body temperature when exposed to thermal changes in the atmosphere. Environmental changes, such as thermal climate change, have an adverse effect on honeybee survival. Insects can be pre-heat-treated (rapid heat hardening) with a mild heat stressor to induce Hsp gene expression and protect them from future stresses. Sixty accepted mother queen (MQ) larvae at the age of 7 days were selected and divided into two incubation treatment groups (n = 30). The control group (non-heat-treated mother queens, nH-T MQ) was maintained at 34.5 degrees C for 15 min and 70% relative humidity (RH), and the preheat-treated (pre-heat-treated mother queens, pH-T MQ) group was subjected to 41 degrees C for 15 min and 70% RH. To evaluate the effect of larval pre-heat-treatment on thermotolerance acquisition of daughter workers, about 500 workers were collected from brood combs of each treatment group. The worker bees in their cages were incubated in digital-controlled thermo-incubators for 1 h under each of the following temperatures: 35, 40, 45, 50, 55, and 60 degrees C. The expression of Hsp40, Hsp60, Hsp70, HSC70-3, HSC70-4, HSC70-5, Hsp83, and Hsp90 genes in both head and thorax were evaluated by relative quantitative real-time qPCR (RT-qPCR). In general, the pH-T MQ workers exhibited a higher ability to tolerate temperature than nH-T MQ workers under extreme conditions. Furthermore, we reported for the first time that pre-heat treatment of the mother queen's larvae alters the basal and dynamic expression of heat shock proteins (Hsp40, Hsp60, Hsp70, and Hsp90) and heat shock factor (HSF) during thermoneutral conditions and during heat stress of daughter workers, probably indicating a substantial improvement of honeybees' thermotolerance acquisition in arid and semi-arid regions, and improvement of honeybee longevity and management strategies.
英文关键词	Dynamics Heat shock protein Heat stress Pre-heat-treatment Apis mellifera
类型	Article
语种	英语
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:000761258400001
WOS关键词	HONEY-BEE ; TEMPERATURE EXTREMES ; MOLECULAR CHAPERONES ; TRANSCRIPTION FACTOR ; HSP70 EXPRESSION ; GENE-EXPRESSION ; THERMAL-STRESS ; CHILL COMA ; DROSOPHILA ; RECOVERY
WOS类目	Biology ; Zoology
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Zoology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393622
推荐引用方式 GB/T 7714	Al-Ghzawi, Abd Al-Majeed A.,Al-Zghoul, Mohammad Borhan,Zaitoun, Shahera,et al. Dynamics of heat shock proteins and heat shock factor expression during heat stress in daughter workers in pre-heat-treated (rapid heat hardening) Apis mellifera mother queens[J],2022,104.
APA	Al-Ghzawi, Abd Al-Majeed A.,Al-Zghoul, Mohammad Borhan,Zaitoun, Shahera,Al-Omary, Ilham Mustafa,&Alahmad, Nour Alhoda.(2022).Dynamics of heat shock proteins and heat shock factor expression during heat stress in daughter workers in pre-heat-treated (rapid heat hardening) Apis mellifera mother queens.JOURNAL OF THERMAL BIOLOGY,104.
MLA	Al-Ghzawi, Abd Al-Majeed A.,et al."Dynamics of heat shock proteins and heat shock factor expression during heat stress in daughter workers in pre-heat-treated (rapid heat hardening) Apis mellifera mother queens".JOURNAL OF THERMAL BIOLOGY 104(2022).