干旱区生态环境知识资源中心(Arid): Determination of higenamine and tretoquinol in spices by solid phase extraction-isotope internal standard-high performance liquid chromatography-tandem mass spectrometry

Arid

DOI	10.1016/j.jfca.2022.104524
	Determination of higenamine and tretoquinol in spices by solid phase extraction-isotope internal standard-high performance liquid chromatography-tandem mass spectrometry
	Yan, Yonghuan; Lian, Kaoqi; Lv, Pin; Ai, Lianfeng; Zhang, Haichao; Zhang, Yan
通讯作者	Zhang, Y
来源期刊	JOURNAL OF FOOD COMPOSITION AND ANALYSIS
ISSN	0889-1575
EISSN	1096-0481
出版年	2022
卷号	110
英文摘要	A method was developed and validated for the determination of two plant-derived doping agents, higenamine (HG) and tretoquinol (TQ), in 26 kinds of spices. This was undertaken using ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and isotope internal standards. The samples were extracted with ethanol containing 0.5% formic acid and purified using an Oasis MCX solid phase extraction cartridge. Subsequently, a C18 column was used for chromatographic separation using methanol and 0.05% formic acid aqueous solution as the mobile phase. UPLC-MS/MS in positive ESI and multiple reaction monitoring mode with HG-D4 and TQ-D9 as the internal standards were used for detection and quantification of analytes. The method was successfully calibrated in the range of 0.2-50 ng mL(-1). The limit of detections (LODs) and limit of quantifications (LOQs) of HG were 0.57-0.88 mu g kg(-1) and 1.80-2.72 mu g kg (-1), respectively; the LODs and LOQs of TQ were 0.39-0.58 mu g kg(-1) and 1.27-1.88 mu g kg(-1), respectively. The average recoveries of the analytes fortified at four levels ranged from 77.1% to 98.7%, with a relative standard deviation < 15%. The proposed method has been successfully used to monitor real samples, and shown to be sensitive, rapid, and convenient. Hence, this method could be used for regulatory purposes to screen for the presence of HG and TQ in various spices.
英文关键词	Plant-derived doping agent Higenamine Tretoquinol UPLC-MS/MS Solid phase extraction Spices
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000806546300009
WOS关键词	QUECHERS
WOS类目	Chemistry, Applied ; Food Science & Technology
WOS研究方向	Chemistry ; Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393434
推荐引用方式 GB/T 7714	Yan, Yonghuan,Lian, Kaoqi,Lv, Pin,et al. Determination of higenamine and tretoquinol in spices by solid phase extraction-isotope internal standard-high performance liquid chromatography-tandem mass spectrometry[J],2022,110.
APA	Yan, Yonghuan,Lian, Kaoqi,Lv, Pin,Ai, Lianfeng,Zhang, Haichao,&Zhang, Yan.(2022).Determination of higenamine and tretoquinol in spices by solid phase extraction-isotope internal standard-high performance liquid chromatography-tandem mass spectrometry.JOURNAL OF FOOD COMPOSITION AND ANALYSIS,110.
MLA	Yan, Yonghuan,et al."Determination of higenamine and tretoquinol in spices by solid phase extraction-isotope internal standard-high performance liquid chromatography-tandem mass spectrometry".JOURNAL OF FOOD COMPOSITION AND ANALYSIS 110(2022).