干旱区生态环境知识资源中心(Arid): Development of a UPLC-FLD method for quantitative analysis of three tetracyclines and two fluoroquinolones in chicken muscle

Arid

DOI	10.1016/j.jfca.2022.104471
	Development of a UPLC-FLD method for quantitative analysis of three tetracyclines and two fluoroquinolones in chicken muscle
	He, Zhaoyuan; Guo, Yawen; Chen, Lan; Xie, Kaizhou; Lu, Yang; Chen, Jinyuan; Zhang, Genxi; Zhang, Tao; Dai, Guojun
通讯作者	Xie, KZ
来源期刊	JOURNAL OF FOOD COMPOSITION AND ANALYSIS
ISSN	0889-1575
EISSN	1096-0481
出版年	2022
卷号	109
英文摘要	A sensitive, rapid and efficient ultra-performance liquid chromatography-fluorescence detection (UPLC-FLD) method was established for detecting tetracycline (TC), oxytetracycline (OTC), doxycycline (DOX), enrofloxacin (ENR) and ciprofloxacin (CIP) residues in chicken muscle. Moreover, the fluorescence detector output signals were processed by dual-channel detection. The samples were extracted with a mixture of acetonitrile and 0.1 M citric acid containing 100 mM magnesium chloride (1:1, V/V, adjusted the pH to 5.0 with ammonia) using liquid-liquid extraction (LLE) and purified by Oasis PRiME hydrophilic-lipophilic balance (HLB) solid-phase extraction (SPE) cartridges (60 mg/3 mL). The target compounds were separated on an ACQUITY UPLC BEH C18 (100 mm x 2.1 mm, 1.7 mu m) chromatographic column using a mobile phase composed of acetonitrile and 0.1 M malonic acid solution containing 50 mM magnesium chloride hexahydrate, and the pH was adjusted to 5.5 by adding ammonia. The limits of detection (LODs) and limits of quantification (LOQs) of the five target compounds in chicken muscle were 0.1-13.1 mu g/kg and 0.4-39.4 mu g/kg, respectively. The recoveries of the five target com-pounds in chicken muscles were above 87.33 % when the spiked concentrations were at the LOQ, 0.5 maximum residue limit (MRL), 1.0 MRL and 2.0 MRL. The intraday relative standard deviations (RSDs) were less than 4.91 %, and the interday RSDs were less than 5.13 %. Ultimately, this novel detection method was successfully applied to the quantitative analysis of TC, OTC, DOX, ENR and CIP in 20 commercial chicken samples from local supermarkets.
英文关键词	Chicken muscle Tetracyclines Fluoroquinolones LLE-SPE UPLC-FLD
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000783380100009
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; MULTI-RESIDUE DETERMINATION ; VETERINARY DRUGS ; FISHERY PRODUCTS ; ANTIBIOTICS ; MEAT ; EXTRACTION ; STABILITY ; VALIDATION ; GROWTH
WOS类目	Chemistry, Applied ; Food Science & Technology
WOS研究方向	Chemistry ; Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393433
推荐引用方式 GB/T 7714	He, Zhaoyuan,Guo, Yawen,Chen, Lan,et al. Development of a UPLC-FLD method for quantitative analysis of three tetracyclines and two fluoroquinolones in chicken muscle[J],2022,109.
APA	He, Zhaoyuan.,Guo, Yawen.,Chen, Lan.,Xie, Kaizhou.,Lu, Yang.,...&Dai, Guojun.(2022).Development of a UPLC-FLD method for quantitative analysis of three tetracyclines and two fluoroquinolones in chicken muscle.JOURNAL OF FOOD COMPOSITION AND ANALYSIS,109.
MLA	He, Zhaoyuan,et al."Development of a UPLC-FLD method for quantitative analysis of three tetracyclines and two fluoroquinolones in chicken muscle".JOURNAL OF FOOD COMPOSITION AND ANALYSIS 109(2022).