干旱区生态环境知识资源中心(Arid): Design and synthesis of amphiphilic carboxyl-functionalized magnetic polymer microspheres for fast determination of paraquat and its four metabolites in human urine samples prior to ultra-high performance liquid chromatography-high resolution mass spectrometry

Arid

DOI	10.1016/j.chroma.2022.462998
	Design and synthesis of amphiphilic carboxyl-functionalized magnetic polymer microspheres for fast determination of paraquat and its four metabolites in human urine samples prior to ultra-high performance liquid chromatography-high resolution mass spectrometry
	Pan, Shengdong; Huang, Zhiping; Shang, Chunqing; Wang, Li; Qiu, Qiaoli; Xu, Zemin; Zhang, Dandan
通讯作者	Pan, SD ; Zhang, DD
来源期刊	JOURNAL OF CHROMATOGRAPHY A
ISSN	0021-9673
EISSN	1873-3778
出版年	2022
卷号	1670
英文摘要	In cases of low concentration paraquat (PQ) poisoning, as the disease progresses over a long period of time, the prototype PQ may not be detected in the urine, which has a significant negative impact on the precise treatment of the poisoning. But PQ poisoning can also be confirmed by the detection of specific metabolites of PQ in the urine samples. In the present study, core-shell amphiphilic carboxyl-functionalized magnetic polymer microsphere (Amphiphilic-MPs-COOH) was prepared, and the as-prepared Amphiphilic-MPs-COOH was characterized by transmission electron microscope (TEM), scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and thermogravimetry and differential thermogravimetry analyses (TG-DTG). Then, the Amphiphilic-MPs-COOH was employed as a magnetic solid-phase extraction (MSPE) adsorbent for pretreatment and rapid determination of PQ and its four metabolites from urine samples prior to ultra-high performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS). The extraction elution conditions of Amphiphilic-MPs-COOH towards PQ and its metabolites were optimized in spiking urine samples to obtain the best MSPE efficiency. The adsorption mechanism of PQ and its metabolites by Amphiphilic-MPs-COOH involves electrostatic attraction and pi-pi stacking interactions. Moreover, the effect of different ratios of hydrophilic monomer NVP and functional monomer 4-VBA on the extraction and purification performance of PQ and its metabolites in urine samples. And the results revealed that both hydrophilic monomer and functional monomer were important for the adsorption of PQ and its metabolites, and the addition of the appropriate amount of the hydrophilic monomer NVP can improve the compatibility of the adsorbent with the urine substrate. In addition, this study compared the matrix effect of the Amphiphilic-MPs-COOH based MSPE method and the commercial Waters Oasis WCX SPE method. The results showed that the Amphiphilic-MPs-COOH based MSPE method developed in this paper had better resistance to matrix interference. Under optimal conditions, the recoveries of PQ and its metabolites were ranging from 84.5 to 103%, with relative standard deviations (RSDs) of 1.1-6.3%. While the limits of detection (LODs, S/N >= 3) and limits of quantification (LOQs, S/N >= 9) of the method were in range of 0.1-1.6 mu g/L and 0.3-4.8 mu g/L, respectively. Finally, the established MSPE-UPLC-HRMS method in this study was used to confirm PQ poisoning not only based on detecting PQ prototype, but also on its four metabolites, providing strong technical support for clinical precision treatment. (c) 2022 Elsevier B.V. All rights reserved.
英文关键词	Amphiphilic carboxyl-functionalized magnetic polymer microspheres (Amphiphilic-MPs-COOH) UPLC-HRMS Magnetic-phase extraction (MSPE) Paraquat Metabolites Urine sample
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000790444300004
WOS关键词	SOLID-PHASE MICROEXTRACTION ; EXTRACTION ; OPTIMIZATION ; ADSORPTION ; HERBICIDE ; DIQUAT ; PLASMA ; LIVER
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393357
推荐引用方式 GB/T 7714	Pan, Shengdong,Huang, Zhiping,Shang, Chunqing,et al. Design and synthesis of amphiphilic carboxyl-functionalized magnetic polymer microspheres for fast determination of paraquat and its four metabolites in human urine samples prior to ultra-high performance liquid chromatography-high resolution mass spectrometry[J],2022,1670.
APA	Pan, Shengdong.,Huang, Zhiping.,Shang, Chunqing.,Wang, Li.,Qiu, Qiaoli.,...&Zhang, Dandan.(2022).Design and synthesis of amphiphilic carboxyl-functionalized magnetic polymer microspheres for fast determination of paraquat and its four metabolites in human urine samples prior to ultra-high performance liquid chromatography-high resolution mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1670.
MLA	Pan, Shengdong,et al."Design and synthesis of amphiphilic carboxyl-functionalized magnetic polymer microspheres for fast determination of paraquat and its four metabolites in human urine samples prior to ultra-high performance liquid chromatography-high resolution mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1670(2022).