干旱区生态环境知识资源中心(Arid): Morphogenetic variation and assorted biological activities in true branching Nostocales strains of Cholistan oasis, Pakistan

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DOI	10.1002/jobm.202100640
	Morphogenetic variation and assorted biological activities in true branching Nostocales strains of Cholistan oasis, Pakistan
	Inam, Farooq; Mumtaz, Abdul S.; Kaleem, Muhammad; Sajid, Imran
通讯作者	Mumtaz, AS
来源期刊	JOURNAL OF BASIC MICROBIOLOGY
ISSN	0233-111X
EISSN	1521-4028
出版年	2022
卷号	62 期号:5 页码:634-643
英文摘要	From the oasis of Cholistan, true branching heterocystous cyanobacterial strains were studied for, the cell arrangement in primary branches being mono- or bi-seriate; the shape of cells in main filament large and irregular; profused secondary branching emerging on one or both sides and tapering along their length. In these observed traits, two clear morphological taxa were recognized, both well-assorted from the previously described species of the genus Westiellopsis. Both strains showed culturing responses and were studied for antibacterial, cytotoxic, and anticancer potentials. The strain derived from the site B-10 provenance exhibited antibacterial activity against Pseudomonas (18 mm), Klebsiella (15 mm), Staphylococcus (22 mm). On the contrary, the strain of site A-44 showed no activity against any of the above-mentioned bacterial strains. The cytotoxicity assay for the strain of the B-10 site showed 36% larval mortality, whereas strain A-44 showed 24% larval mortality. Performance of the strain B-10 in MTT assay (assessed on HCT-116 cell lines) revealed a dose-dependent activity: at 200, 100, 50, and 25 mu g/ml; achieving a growth inhibition of 50.15%, 40.22%, 33.72%, and 10.21%, respectively; and the strain of A-44 could only exhibit a 30.06% growth inhibition at 200 mu g/ml. The 16S rRNA sequencing revealed the sequence homology with Neowestiellopsis. Based on data presented here we report two diverse taxa of true branching Nostocales from Cholistan oasis, Pakistan.
英文关键词	16S rRNA Cholistan Cyanobacteria morphology
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000774529500001
WOS关键词	BRINE SHRIMP ; CYANOBACTERIA ; CYANOPHYTA ; CLASSIFICATION ; GENERA ; ASSAY
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393343
推荐引用方式 GB/T 7714	Inam, Farooq,Mumtaz, Abdul S.,Kaleem, Muhammad,et al. Morphogenetic variation and assorted biological activities in true branching Nostocales strains of Cholistan oasis, Pakistan[J],2022,62(5):634-643.
APA	Inam, Farooq,Mumtaz, Abdul S.,Kaleem, Muhammad,&Sajid, Imran.(2022).Morphogenetic variation and assorted biological activities in true branching Nostocales strains of Cholistan oasis, Pakistan.JOURNAL OF BASIC MICROBIOLOGY,62(5),634-643.
MLA	Inam, Farooq,et al."Morphogenetic variation and assorted biological activities in true branching Nostocales strains of Cholistan oasis, Pakistan".JOURNAL OF BASIC MICROBIOLOGY 62.5(2022):634-643.