干旱区生态环境知识资源中心(Arid): Improvement of plant tolerance to drought stress by cotton tubby-like protein 30 through stomatal movement regulation

Arid

DOI	10.1016/j.jare.2022.06.007
	Improvement of plant tolerance to drought stress by cotton tubby-like protein 30 through stomatal movement regulation
	Li, Zhanshuai; Liu, Ji; Kuang, Meng; Zhang, Chaojun; Ma, Qifeng; Huang, Longyu; Wang, Huiying; Fan, Shuli; Peng, Jun
通讯作者	Fan, SL ; Peng, J
来源期刊	JOURNAL OF ADVANCED RESEARCH
ISSN	2090-1232
EISSN	2090-1224
出版年	2022
卷号	42 页码:55-67
英文摘要	Introduction: Cotton is a vital industrial crop that is gradually shifting to planting in arid areas. However, tubby-like proteins (TULPs) involved in plant response to various stresses are rarely reported in cotton. The present study exhibited that GhTULP30 transcription in cotton was induced by drought stress. Objective: The present study demonstrated the improvement of plant tolerance to drought stress by GhTULP30 through regulation of stomatal movement.Methods: GhTULP30 response to drought and salt stress was preliminarily confirmed by qRT-PCR and yeast stress experiments. Ectopic expression in Arabidopsis and endogenous gene silencing in cotton were used to determine stomatal movement. Yeast two-hybrid and spilt-luciferase were used to screen the interacting proteins.Results: Ectopic expression of GhTULP30 in yeast markedly improved yeast cell tolerance to salt and drought. Overexpression of GhTULP30 made Arabidopsis seeds more resistant to drought and salt stress during seed germination and increased the stomata closing speed of the plant under drought stress con-ditions. Silencing of GhTULP30 in cotton by virus-induced gene silencing (VIGS) technology slowed down the closure speed of stomata under drought stress and decreased the length and width of the stomata.The trypan blue and diaminobenzidine staining exhibited the severity of leaf cell necrosis of GhTULP30- silenced plants. Additionally, the contents of proline, malondialdehyde, and catalase of GhTULP30- silenced plants exhibited significant variations, with obvious leaf wilting. Protein interaction experiments exhibited the interaction of GhTULP30 with GhSKP1B and GhXERICO.Conclusion: GhTULP30 participates in plant response to drought stress. The present study provides a ref-erence and direction for further exploration of TULP functions in cotton plants.(c) 2022 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
英文关键词	Cotton GhTULP30 Drought Seed germination Stomatal movement
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000906627500001
WOS关键词	TRANSCRIPTION FACTOR ; BIOTIC STRESS ; ARABIDOPSIS ; GENE ; ACID ; IDENTIFICATION ; TEMPERATURE ; UBIQUITIN ; GROWTH ; MEMBER
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393258
推荐引用方式 GB/T 7714	Li, Zhanshuai,Liu, Ji,Kuang, Meng,et al. Improvement of plant tolerance to drought stress by cotton tubby-like protein 30 through stomatal movement regulation[J],2022,42:55-67.
APA	Li, Zhanshuai.,Liu, Ji.,Kuang, Meng.,Zhang, Chaojun.,Ma, Qifeng.,...&Peng, Jun.(2022).Improvement of plant tolerance to drought stress by cotton tubby-like protein 30 through stomatal movement regulation.JOURNAL OF ADVANCED RESEARCH,42,55-67.
MLA	Li, Zhanshuai,et al."Improvement of plant tolerance to drought stress by cotton tubby-like protein 30 through stomatal movement regulation".JOURNAL OF ADVANCED RESEARCH 42(2022):55-67.