干旱区生态环境知识资源中心(Arid): Ontogenetic Variation in Macrocyclic and Hemicyclic Poplar Rust Fungi

Arid

DOI	10.3390/ijms232113062
	Ontogenetic Variation in Macrocyclic and Hemicyclic Poplar Rust Fungi
	Yu, Zhongdong; Peng, Zijia; Qi, Mei; Zheng, Wei
通讯作者	Yu, ZD
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN	1422-0067
出版年	2022
卷号	23 期号:21
英文摘要	Melampsora larici-populina (Mlp), M. medusae (Mmed), M. magnusiana (Mmag), and M. pruinosae (Mpr) are epidemic rust fungi in China. The first two are macrocyclic rust fungi distributed in temperate humid environments. The latter two are hemicyclic rusts, mainly distributed in arid and semi-arid areas. Ontogenetic variation that comes with this arid-resistance is of great interest-and may help us predict the influence of a warmer, drier, climate on fungal phylogeny. To compare the differences in the life history and ontogeny between the two types of rust, we cloned mating type genes, STE3.4 and STE3.3 using RACE-smart technology. Protein structures, functions, and mutant loci were compared across each species. We also used microscopy to compare visible cytological differences at each life stage for the fungal species, looking for variation in structure and developmental timing. Quantitative PCR technology was used to check the expression of nuclear fusion and division genes downstream of STE3.3 and STE3.4. Encoding amino acids of STE3.3 and STE3.4 in hemicyclic rusts are shorter than these in the macrocyclic rusts. Both STE3.3 and STE3.4 interact with a protein kinase superfamily member EGG12818 and an E3 ubiquitin protein ligase EGG09709 directly, and activating G-beta conformational changes. The mutation at site 74th amino acid in the conserved transmembrane domain of STE3.3 ascribes to a positive selection, in which alanine (Ala) is changed to phenylalanine (Phe) in hemicyclic rusts, and a mutation with Tyr lost at site 387th in STE3.4, where it is the binding site for beta-D-Glucan. These mutants are speculated corresponding to the insensitivity of hemicyclic rust pheromone receptors to interact with MFa pheromones, and lead to Mnd1 unexpressed in teliospora, and they result in the diploid nuclei division failure and the sexual stage missing in the life cycle. A Phylogenic tree based on STE3.4 gene suggests these two rust types diverged about 14.36 million years ago. Although these rusts share a similar uredia and telia stage, they show markedly different wintering strategies. Hemicyclic rusts overwinter in the poplar buds endophytically, their urediniospores developing thicker cell walls. They form haustoria with a collar-like extrahaustorial membrane neck and induce host thickened callose cell walls, all ontogenetic adaptations to arid environments.
英文关键词	Melampsora mating type gene ontogeny macrocyclic hemicyclic
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000881298700001
WOS关键词	MELAMPSORA-LARICI-POPULINA ; MATING-TYPE ; IDENTIFICATION ; GENES ; RECOGNITION ; EVOLUTION ; REVEALS ; CASCADE ; LINKAGE ; GROWTH
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393202
推荐引用方式 GB/T 7714	Yu, Zhongdong,Peng, Zijia,Qi, Mei,et al. Ontogenetic Variation in Macrocyclic and Hemicyclic Poplar Rust Fungi[J],2022,23(21).
APA	Yu, Zhongdong,Peng, Zijia,Qi, Mei,&Zheng, Wei.(2022).Ontogenetic Variation in Macrocyclic and Hemicyclic Poplar Rust Fungi.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(21).
MLA	Yu, Zhongdong,et al."Ontogenetic Variation in Macrocyclic and Hemicyclic Poplar Rust Fungi".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.21(2022).