干旱区生态环境知识资源中心(Arid): miR2119, a Novel Transcriptional Regulator, Plays a Positive Role in Woody Plant Drought Tolerance by Mediating the Degradation of the CkBI-1 Gene Associated with Apoptosis

Arid

DOI	10.3390/ijms23116306
	miR2119, a Novel Transcriptional Regulator, Plays a Positive Role in Woody Plant Drought Tolerance by Mediating the Degradation of the CkBI-1 Gene Associated with Apoptosis
	Liu, Furong; Zhang, Puzhi; Li, Jiayang; Zhang, Tianxin; Xie, Lifang; Gong, Chunmei
通讯作者	Gong, CM
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN	1422-0067
出版年	2022
卷号	23 期号:11
英文摘要	Caragana korshinskii, an important vegetation restoration species with economic and ecological benefits in the arid region of northwest China, is characterized by significant drought tolerance. However, the underlying molecular mechanisms by which miRNAs confer this trait in C. korshinskii are unclear. Here, we investigate the effect of CkmiR2119 on drought tolerance and identified its target gene, CkBI-1. A negative correlation of CkmiR2119 and CkBI-1 in both stems and leaves in a drought gradient treatment followed by target gene validation suggest that CkmiR2119 might negatively regulate CkBI-1. Consistently, a decrease in the expression of the CkBI-1 gene was observed after both transient transformation and stable transformation of CkamiR2119 in tobacco (Nicotiana tabacum). Moreover, the physiological analysis of CkamiR2119 and CkBI-1 transgenic plants further indicate that CkmiR2119 can enhance the drought tolerance of C. korshinskii in two aspects: (i) downregulating CkBI-1 expression to accelerate vessel maturation in stems; (ii) contributing to a higher level of CkBI-1 in mesophyll cells to inhibit programmed cell death (PCD). This work reveals that CkmiR2119 can increase plants' drought tolerance by downregulating the expression of CkBI-1, providing a theoretical basis to improve plants' ability to withstand stress tolerance by manipulating miRNAs.
英文关键词	Caragana korshinskii drought CkamiR2119 CkBI-1
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000808919800001
WOS关键词	PROGRAMMED CELL-DEATH ; BAX INHIBITOR-1 ; SMALL RNAS ; STRESS-RESPONSE ; MICRORNAS ; ARABIDOPSIS ; SUPPRESSOR ; TRIGGERS ; PATHWAY
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393197
推荐引用方式 GB/T 7714	Liu, Furong,Zhang, Puzhi,Li, Jiayang,et al. miR2119, a Novel Transcriptional Regulator, Plays a Positive Role in Woody Plant Drought Tolerance by Mediating the Degradation of the CkBI-1 Gene Associated with Apoptosis[J],2022,23(11).
APA	Liu, Furong,Zhang, Puzhi,Li, Jiayang,Zhang, Tianxin,Xie, Lifang,&Gong, Chunmei.(2022).miR2119, a Novel Transcriptional Regulator, Plays a Positive Role in Woody Plant Drought Tolerance by Mediating the Degradation of the CkBI-1 Gene Associated with Apoptosis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(11).
MLA	Liu, Furong,et al."miR2119, a Novel Transcriptional Regulator, Plays a Positive Role in Woody Plant Drought Tolerance by Mediating the Degradation of the CkBI-1 Gene Associated with Apoptosis".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.11(2022).