干旱区生态环境知识资源中心(Arid): Chemical Fingerprinting of Heat Stress Responses in the Leaves of Common Wheat by Fourier Transform Infrared Spectroscopy

Arid

DOI	10.3390/ijms23052842
	Chemical Fingerprinting of Heat Stress Responses in the Leaves of Common Wheat by Fourier Transform Infrared Spectroscopy
	Osman, Salma O. M.; Saad, Abu Sefyan I.; Tadano, Shota; Takeda, Yoshiki; Konaka, Takafumi; Yamasaki, Yuji; Tahir, Izzat S. A.; Tsujimoto, Hisashi; Akashi, Kinya
通讯作者	Akashi, K
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN	1422-0067
出版年	2022
卷号	23 期号:5
英文摘要	Wheat (Triticum aestivum L.) is known to be negatively affected by heat stress, and its production is threatened by global warming, particularly in arid regions. Thus, efforts to better understand the molecular responses of wheat to heat stress are required. In the present study, Fourier transform infrared (FTIR) spectroscopy, coupled with chemometrics, was applied to develop a protocol that monitors chemical changes in common wheat under heat stress. Wheat plants at the three-leaf stage were subjected to heat stress at a 42 degrees C daily maximum temperature for 3 days, and this led to delayed growth in comparison to that of the control. Measurement of FTIR spectra and their principal component analysis showed partially overlapping features between heat-stressed and control leaves. In contrast, supervised machine learning through linear discriminant analysis (LDA) of the spectra demonstrated clear discrimination of heat-stressed leaves from the controls. Analysis of LDA loading suggested that several wavenumbers in the fingerprinting region (400-1800 cm(-1)) contributed significantly to their discrimination. Novel spectrum-based biomarkers were developed using these discriminative wavenumbers that enabled the successful diagnosis of heat-stressed leaves. Overall, these observations demonstrate the versatility of FTIR-based chemical fingerprints for use in heat-stress profiling in wheat.
英文关键词	wheat heat stress FTIR spectroscopy chemometrics arid region linear discriminant analysis spectral biomarker
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000768786100001
WOS关键词	ANTIOXIDATIVE DEFENSE SYSTEM ; FTIR SPECTROSCOPY ; CARBOHYDRATE-CHEMISTRY ; HIGH-TEMPERATURE ; WATER-STRESS ; ATR-FTIR ; MICROSPECTROSCOPY
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393196
推荐引用方式 GB/T 7714	Osman, Salma O. M.,Saad, Abu Sefyan I.,Tadano, Shota,et al. Chemical Fingerprinting of Heat Stress Responses in the Leaves of Common Wheat by Fourier Transform Infrared Spectroscopy[J],2022,23(5).
APA	Osman, Salma O. M..,Saad, Abu Sefyan I..,Tadano, Shota.,Takeda, Yoshiki.,Konaka, Takafumi.,...&Akashi, Kinya.(2022).Chemical Fingerprinting of Heat Stress Responses in the Leaves of Common Wheat by Fourier Transform Infrared Spectroscopy.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(5).
MLA	Osman, Salma O. M.,et al."Chemical Fingerprinting of Heat Stress Responses in the Leaves of Common Wheat by Fourier Transform Infrared Spectroscopy".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.5(2022).