干旱区生态环境知识资源中心(Arid): Biomarkers in the Atacama Desert along the moisture gradient and the depth in the hyperarid zone: Phosphatase activity as trace of microbial activity

Arid

DOI	10.1017/S1473550422000325
	Biomarkers in the Atacama Desert along the moisture gradient and the depth in the hyperarid zone: Phosphatase activity as trace of microbial activity
	Kobayashi, Kensei; Nauny, Philippe; Takano, Yoshinori; Honma, Chiho; Kurizuka, Taihei; Ishikawa, Yuto; Yogosawa, Shusuke; Obayashi, Yumiko; Kaneko, Takeo; Kebukawa, Yoko; Mita, Hajime; Ogawa, Mari; Enya, Keigo; Yoshimura, Yoshitaka; McKay, Christopher P.
通讯作者	Kobayashi, K
来源期刊	INTERNATIONAL JOURNAL OF ASTROBIOLOGY
ISSN	1473-5504
EISSN	1475-3006
出版年	2022
卷号	21 期号:5 页码:329-351
英文摘要	Microbiological activities can be detected in various extreme environments on Earth, which suggest that extraterrestrial environments, such as on Mars, could host life. There have been proposed a number of biomarkers to detect extant life mostly based on specific molecules. Because terrestrial organisms have catalytic proteins (enzymes), enzymatic activity may also be a good indicator to evaluate biological activities in extreme environments. Phosphatases are essential for all terrestrial organisms because phosphate esters are ubiquitously used in genetic molecules (DNA/RNA) and membranes. In this study, we evaluated microbial activity in soils of the Atacama Desert, Chile, by analysing several biomarkers, including phosphatase activity. Phosphatases extracted with Tris buffer were assayed fluorometrically using 4-methylumbelliferyl phosphate as a substrate. The horizontal distribution of phosphatase activity and other parameters in soils from the Atacama Desert showed that phosphatase activity was positively correlated with amino acid concentration and colony-forming units and negatively correlated with precipitation amount. We found consistent that biochemical indicators including phosphatase significantly decreased in the extreme hyper-arid zone where rainfall of <25 mm year(-1). The results were compared with phosphatase activities detected in extreme environments, such as submarine hydrothermal systems and Antarctic soils, as well as soils from ordinary environments. Overall, our results suggested that phosphatase activity could be a good indicator for evaluating biological activities in extreme environments.
英文关键词	Amino acids Atacama Desert biomarkers extant life life detection lipids Mars phosphatase activity
类型	Article
语种	英语
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:000844871400001
WOS关键词	IZU-BONIN ARC ; MARS-LIKE SOILS ; AMINO-ACIDS ; SUIYO SEAMOUNT ; ALKALINE-PHOSPHATASE ; LIFE ; DEEP ; COMPLEX ; ORIGIN ; ENVIRONMENTS
WOS类目	Astronomy & Astrophysics ; Biology ; Geosciences, Multidisciplinary
WOS研究方向	Astronomy & Astrophysics ; Life Sciences & Biomedicine - Other Topics ; Geology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393123
推荐引用方式 GB/T 7714	Kobayashi, Kensei,Nauny, Philippe,Takano, Yoshinori,et al. Biomarkers in the Atacama Desert along the moisture gradient and the depth in the hyperarid zone: Phosphatase activity as trace of microbial activity[J],2022,21(5):329-351.
APA	Kobayashi, Kensei.,Nauny, Philippe.,Takano, Yoshinori.,Honma, Chiho.,Kurizuka, Taihei.,...&McKay, Christopher P..(2022).Biomarkers in the Atacama Desert along the moisture gradient and the depth in the hyperarid zone: Phosphatase activity as trace of microbial activity.INTERNATIONAL JOURNAL OF ASTROBIOLOGY,21(5),329-351.
MLA	Kobayashi, Kensei,et al."Biomarkers in the Atacama Desert along the moisture gradient and the depth in the hyperarid zone: Phosphatase activity as trace of microbial activity".INTERNATIONAL JOURNAL OF ASTROBIOLOGY 21.5(2022):329-351.