干旱区生态环境知识资源中心(Arid): Investigation of the Sandfly Fauna of Central Arid Areas and Northern Humid Regions of Tunisia, with Morphological and Molecular Identification of the Recently Established Population of Phlebotomus (Larroussius) perfiliewi

Arid

DOI	10.3390/insects13111057
	Investigation of the Sandfly Fauna of Central Arid Areas and Northern Humid Regions of Tunisia, with Morphological and Molecular Identification of the Recently Established Population of Phlebotomus (Larroussius) perfiliewi
	Chelbi, Ifhem; Abdi, Amani; Depaquit, Jerome; Fares, Wasfi; Abbas, Mohammed Abdo Saghir; Dachraoui, Khalil; Zhioua, Elyes
通讯作者	Zhioua, E
来源期刊	INSECTS
EISSN	2075-4450
出版年	2022
卷号	13 期号:11
英文摘要	Simple Summary Phlebotomus perfiliewi is one of the main vectors of Leishmania infantum, etiologic agent of zoonotic visceral leishmaniasis (ZVL), in the Western Mediterranean basin including Tunisia. Phlebotomus perfiliewi, originally endemic only in Northern humid bio-climatic zones, became established in a highly irrigated area of the central arid bioclimatic zone. Therefore, surveillance of sandfly vectors is a cornerstone for the establishment of control strategies. An entomological survey was carried out using light traps in a humid and in a highly irrigated arid bio-climatic zone located in northern and central Tunisia, respectively. Collected sandflies were identified based on morphological criteria, and identification of Ph. perfiliewi was confirmed by molecular tools. Results indicated that Ph. perfiliewi is the most abundant sandfly species in both sites and it is genetically variable. Thus, surveillance and control of sandflies is highly needed to limit the incidence of sandfly-borne diseases. Based on nucleotide sequences, we re-identified representative samples of Phlebotomus perfiliewi originating from two different biogeographical areas of Tunisia, whose populations had previously been identified based on morphological criteria. A partial region of the mitochondrial DNA cytochrome b gene was targeted, and sandfly species was determined by analogy with DNA sequences available in the GenBank database via a BLAST analysis, taking into account the query coverage and percentage identity. The recognized species presents the most substantial homology with the analyzed sequence. The results of the molecular identification showed complete agreement with the morphological identifications, and Phlebotomus perfiliewi is genetically variable.
英文关键词	Phlebotomus perfiliewi morphological identification molecular identification bioclimatic areas zoonotic visceral leishmaniasis
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000895362600001
WOS关键词	ZOONOTIC CUTANEOUS LEISHMANIASIS ; VISCERAL LEISHMANIASIS ; DIPTERA ; PSYCHODIDAE ; VECTORS ; PARROT ; FOCUS ; DIFFERENTIATION ; CONSEQUENCES ; TRANSMISSION
WOS类目	Entomology
WOS研究方向	Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/393107
推荐引用方式 GB/T 7714	Chelbi, Ifhem,Abdi, Amani,Depaquit, Jerome,et al. Investigation of the Sandfly Fauna of Central Arid Areas and Northern Humid Regions of Tunisia, with Morphological and Molecular Identification of the Recently Established Population of Phlebotomus (Larroussius) perfiliewi[J],2022,13(11).
APA	Chelbi, Ifhem.,Abdi, Amani.,Depaquit, Jerome.,Fares, Wasfi.,Abbas, Mohammed Abdo Saghir.,...&Zhioua, Elyes.(2022).Investigation of the Sandfly Fauna of Central Arid Areas and Northern Humid Regions of Tunisia, with Morphological and Molecular Identification of the Recently Established Population of Phlebotomus (Larroussius) perfiliewi.INSECTS,13(11).
MLA	Chelbi, Ifhem,et al."Investigation of the Sandfly Fauna of Central Arid Areas and Northern Humid Regions of Tunisia, with Morphological and Molecular Identification of the Recently Established Population of Phlebotomus (Larroussius) perfiliewi".INSECTS 13.11(2022).