干旱区生态环境知识资源中心(Arid): Selection of the Reference Gene for Expression Normalization in Salsola ferganica under Abiotic Stress

Arid

DOI	10.3390/genes13040571
	Selection of the Reference Gene for Expression Normalization in Salsola ferganica under Abiotic Stress
	Wang, Shuran; Zhang, Sheng
通讯作者	Zhang, S
来源期刊	GENES
EISSN	2073-4425
出版年	2022
卷号	13 期号:4
英文摘要	Salsola ferganica is a natural desert herbaceous plant in the arid area of western and northwestern China. Because of its salt tolerance and drought resistance, it is of great significance in desert afforestation and sand-fixing capacity. There has been much research on the genes involved in plants under desert stresses in recent years. The application of the best internal reference genes for standardization was a critical procedure in analyzing the gene expression under different types. Even so, the reference gene has not been reported in the application of gene expression normalization of S. ferganica. In this study, nine reference genes (TUA-1726, TUA-1760, TUB, GAPDH, ACT, 50S, HSC70, APT, and U-box) in S. ferganica were adopted and analyzed under six different treatments (ABA, heat, cold, NaCl, methyl viologen (MV), and PEG). The applicability of candidate genes was evaluated by statistical software, including geNorm, NormFinder, BestKeeper, and RefFinder, based on their stability values in all the treatments. These results indicated that the simultaneous selection of two stable reference genes would fully standardize the optimization of the normalization research. To verify the feasibility of the above internal reference genes, the CT values of AP2/ERF transcription factor family genes were standardized using the most (ACT) and least (GAPDH) stable reference genes in S. ferganica seedlings under six abiotic stresses. The research showed that HSC70 and U-box were the most appropriate reference genes in ABA stressed samples, and ACT and U-box genes were the optimal references for heat-stressed samples. TUA-1726 and U-box showed the smallest value in gene expression levels of cold treatment. The internal reference groups of the best applicability for the other samples were U-box and ACT under NaCl treatment, ACT and TUA-1726 under MV stress, HSC70 and TUB under PEG treatment, and ACT in all samples. ACT and U-box showed higher stability than the other genes based on the comprehensive stability ranking of RefFinder, as determined by the geometric mean in this study. These results will contribute to later gene expression studies in other closely related species and provide an important foundation for gene expression analysis in S. ferganica.
英文关键词	Salsola ferganica abiotic stress reference gene qRT-PCR
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000787446800001
WOS关键词	SUITABLE REFERENCE GENES ; REAL-TIME PCR ; VALIDATION
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392886
推荐引用方式 GB/T 7714	Wang, Shuran,Zhang, Sheng. Selection of the Reference Gene for Expression Normalization in Salsola ferganica under Abiotic Stress[J],2022,13(4).
APA	Wang, Shuran,&Zhang, Sheng.(2022).Selection of the Reference Gene for Expression Normalization in Salsola ferganica under Abiotic Stress.GENES,13(4).
MLA	Wang, Shuran,et al."Selection of the Reference Gene for Expression Normalization in Salsola ferganica under Abiotic Stress".GENES 13.4(2022).