干旱区生态环境知识资源中心(Arid): Transcriptome analysis reveals the main metabolic pathway of c-GMP induced by salt stress in tomato (Solanum lycopersicum) seedlings

Arid

DOI	10.1071/FP21337
	Transcriptome analysis reveals the main metabolic pathway of c-GMP induced by salt stress in tomato (Solanum lycopersicum) seedlings
	Zhu, Xiaolin; Su, Meifei; Wang, Baoqiang; Wei, Xiaohong
通讯作者	Wei, XH
来源期刊	FUNCTIONAL PLANT BIOLOGY
ISSN	1445-4408
EISSN	1445-4416
出版年	2022
卷号	49 期号:9 页码:784-798
英文摘要	Tomato (Solanum lycopersicum L.) is a model crop as well as an important food worldwide. In arid areas, increasing soil salinity has limited higher yields in tomato production. As a second messenger molecule, cyclic guanosine monophosphate (c-GMP) plays an indispensable role in plant response to salt stress by regulating cell processes to promote plant growth and development. However, this mechanism has not been fully explored in tomato seedlings. In this experiment, tomato seeds were cultured in four treatments: (I) distilled water (CK); (2) 20 mu M c-GMP (TI); (3) 50 mM NaCl (T2); and (4) 20 mu M c-GMP + 50 mM NaCl (T3). The results show that 20 mu M c-GMP effectively alleviated the inhibitory effect of 50 mM NaCl on growth and development, and induced the expression of 1580 differentially expressed genes (DEGs). Seedlings in the CK vs TI shared 95 upregulated and 442 downregulated DEGs, whereas T2 vs T3 shared 271 upregulated and 772 down regulated DEGs. Based on KEGG (Kyoto Encyclopaedia of Genes and Genomes) analysis, the majority of DEGs were involved in metabolism; exogenous c-GMP induced significant enrichment of pathways associated with carbohydrates, phenylpropanoids and fatty acid metabolism. Most PMEs, acCoA, PAL, PODs, FADs, and AD were upregulated, and GAPDHs, PL, PG, BXL4, and beta-G were downregulated, which reduced susceptibility of tomato seedlings to salt and promoted their salt tolerance. The application of c-GMP increased soluble sugar, flavonoid and lignin contents, reduced accumulation of malondialdehyde (MDA), and enhanced the activity of peroxidase (POD). Thus, our results provide insights into the molecular mechanisms associated with salt tolerance of tomato seedlings.
英文关键词	c-GMP differentially expressed genes qRT-PCR salt stress secondary metabolites tomato seedlings transcription factor transcriptome sequencing
类型	Article
语种	英语
开放获取类型	Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000791449900001
WOS关键词	GENE-EXPRESSION ; FATTY ALDEHYDE ; ABSCISIC-ACID ; NITRIC-OXIDE ; CYCLIC-GMP ; CELL-WALL ; PLANT ; TOLERANCE ; RESPONSES ; ROLES
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392883
推荐引用方式 GB/T 7714	Zhu, Xiaolin,Su, Meifei,Wang, Baoqiang,et al. Transcriptome analysis reveals the main metabolic pathway of c-GMP induced by salt stress in tomato (Solanum lycopersicum) seedlings[J],2022,49(9):784-798.
APA	Zhu, Xiaolin,Su, Meifei,Wang, Baoqiang,&Wei, Xiaohong.(2022).Transcriptome analysis reveals the main metabolic pathway of c-GMP induced by salt stress in tomato (Solanum lycopersicum) seedlings.FUNCTIONAL PLANT BIOLOGY,49(9),784-798.
MLA	Zhu, Xiaolin,et al."Transcriptome analysis reveals the main metabolic pathway of c-GMP induced by salt stress in tomato (Solanum lycopersicum) seedlings".FUNCTIONAL PLANT BIOLOGY 49.9(2022):784-798.