干旱区生态环境知识资源中心(Arid): Full-length transcriptome sequencing analysis and characterization, development and validation of microsatellite markers in Kengyilia melanthera

Arid

DOI	10.3389/fpls.2022.959042
	Full-length transcriptome sequencing analysis and characterization, development and validation of microsatellite markers in Kengyilia melanthera
	Xiong, Yanli; Yang, Jian; Xiong, Yi; Zhao, Junming; Liu, Lin; Liu, Wei; Sha, Lina; Zhou, Jiqiong; You, Minghong; Li, Daxu; Lei, Xiong; Bai, Shiqie; Ma, Xiao
通讯作者	Ma, X
来源期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
出版年	2022
卷号	13
英文摘要	As a typical psammophyte of the Triticeae, Kengyilia melanthera possesses high feeding potential and great utilization values in desertification control in the Qinghai-Tibet Plateau. However, few gene function and genetic studies have been performed in K. melanthera. In this study, single-molecule real-time sequencing technology was used to obtain the full-length transcriptome sequence of K. melanthera, following the functional annotation of transcripts and prediction of coding sequences (CDSs), transcription factors (TFs), and long noncoding RNA (lncRNA) sequences. Meanwhile, a total of 42,433 SSR loci were detected, with 5 '-UTRs having the most SSR loci and trinucleotide being the most abundant type. In total, 108,399 SSR markers were designed, and 300 SSR markers were randomly selected for diversity verification of K. melanthera. A total of 49 polymorphic SSR markers were used to construct the genetic relationships of 56 K. melanthera accessions, among which 21 SSR markers showed good cross-species transferability among the related species. In conclusion, the full-length transcriptome sequence of the K. melanthera will assist gene prediction and promote molecular biology and genomics research, and the polymorphic SSR markers will promote molecular-assisted breeding and related research of K. melanthera and its relatives.
英文关键词	Kengyilia melanthera full-length transcriptome SMRT sequencing SSRs development transferability
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000838089600001
WOS关键词	MATERNAL DONOR ; PROTEIN ; PROGRAM ; PHYLOGENY ; DATABASE ; TOOL
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392860
推荐引用方式 GB/T 7714	Xiong, Yanli,Yang, Jian,Xiong, Yi,et al. Full-length transcriptome sequencing analysis and characterization, development and validation of microsatellite markers in Kengyilia melanthera[J],2022,13.
APA	Xiong, Yanli.,Yang, Jian.,Xiong, Yi.,Zhao, Junming.,Liu, Lin.,...&Ma, Xiao.(2022).Full-length transcriptome sequencing analysis and characterization, development and validation of microsatellite markers in Kengyilia melanthera.FRONTIERS IN PLANT SCIENCE,13.
MLA	Xiong, Yanli,et al."Full-length transcriptome sequencing analysis and characterization, development and validation of microsatellite markers in Kengyilia melanthera".FRONTIERS IN PLANT SCIENCE 13(2022).