干旱区生态环境知识资源中心(Arid): ACC deaminase-producing endophytic fungal consortia promotes drought stress tolerance in M.oleifera by mitigating ethylene and H2O2

Arid

DOI	10.3389/fpls.2022.967672
	ACC deaminase-producing endophytic fungal consortia promotes drought stress tolerance in M.oleifera by mitigating ethylene and H2O2
	Rehman, Bushra; Javed, Javeria; Rauf, Mamoona; Khan, Sumera Afzal; Arif, Muhammad; Hamayun, Muhammad; Gul, Humaira; Khilji, Sheza Ayaz; Sajid, Zahoor Ahmad; Kim, Won-Chan; Lee, In-Jung
通讯作者	Khan, SA
来源期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
出版年	2022
卷号	13
英文摘要	IntroductionDrought has become more prevalent due to dramatic climate change worldwide. Consequently, the most compatible fungal communities collaborate to boost plant development and ecophysiological responses under environmental constraints. However, little is known about the specific interactions between non-host plants and endophytic fungal symbionts that produce growth-promoting and stress-alleviating hormones during water deficits. MethodsThe current research was rationalized and aimed at exploring the influence of the newly isolated, drought-resistant, ACC deaminase enzyme-producing endophytic fungi Trichoderma gamsii (TP), Fusarium proliferatum (TR), and its consortium (TP+TR) from a xerophytic plant Carthamus oxycantha L. on Moringa oleifera L. grown under water deficit induced by PEG-8000 (8% osmoticum solution). ResultsThe current findings revealed that the co-inoculation promoted a significant enhancement in growth traits such as dry weight (217%), fresh weight (123%), root length (65%), shoot length (53%), carotenoids (87%), and chlorophyll content (76%) in comparison to control plants under water deficit. Total soluble sugars (0.56%), proteins (132%), lipids (43%), flavonoids (52%), phenols (34%), proline (55%), GA(3) (86%), IAA (35%), AsA (170%), SA (87%), were also induced, while H2O2 (-45%), ABA (-60%) and ACC level (-77%) was decreased by co-inoculation of TP and TR in M. oleifera plants, compared with the non-inoculated plants under water deficit. The co-inoculum (TP+TR) also induced the antioxidant potential and enzyme activities POX (325%), CAT activity (166%), and AsA (21%), along with a lesser decrease (-2%) in water potential in M. oleifera plants with co-inoculation under water deficit compared with non-inoculated control. The molecular analysis for gene expression unraveled the reduced expression of ethylene biosynthesis and signaling-related genes up to an optimal level, with an induction of antioxidant enzymatic genes by endophytic co-inoculation in M. oleifera plants under water deficit, suggesting their role in drought stress tolerance as an essential regulatory function. ConclusionThe finding may alert scientists to consider the impacts of optimal reduction of ethylene and induction of antioxidant potential on drought stress tolerance in M. oleifera. Hence, the present study supports the use of compatible endophytic fungi to build a bipartite mutualistic symbiosis in M. oleifera non-host plants to mitigate the negative impacts of water scarcity in arid regions throughout the world.
英文关键词	plant-microbe interaction Moringa oleifera drought stress antioxidants agroforestry hydrogen peroxide ethylene ACC deaminase
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000907682400001
WOS关键词	ABIOTIC STRESS ; GENE-EXPRESSION ; PLANT-GROWTH ; TRICHODERMA ; RESPONSES ; ACCUMULATION ; MAIZE ; BIOSYNTHESIS ; INHIBITION ; BIOCONTROL
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392836
推荐引用方式 GB/T 7714	Rehman, Bushra,Javed, Javeria,Rauf, Mamoona,et al. ACC deaminase-producing endophytic fungal consortia promotes drought stress tolerance in M.oleifera by mitigating ethylene and H2O2[J],2022,13.
APA	Rehman, Bushra.,Javed, Javeria.,Rauf, Mamoona.,Khan, Sumera Afzal.,Arif, Muhammad.,...&Lee, In-Jung.(2022).ACC deaminase-producing endophytic fungal consortia promotes drought stress tolerance in M.oleifera by mitigating ethylene and H2O2.FRONTIERS IN PLANT SCIENCE,13.
MLA	Rehman, Bushra,et al."ACC deaminase-producing endophytic fungal consortia promotes drought stress tolerance in M.oleifera by mitigating ethylene and H2O2".FRONTIERS IN PLANT SCIENCE 13(2022).