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DOI	10.3389/fpls.2022.956298
	Characterization of salt tolerant wheat genotypes by using morpho-physiological, biochemical, and molecular analysis
	Irshad, Ahsan; Ahmed, Rana Imtiaz; Rehman, Shoaib Ur; Sun, Guozhong; Ahmad, Furqan; Sher, Muhammad Ali; Aslam, Muhammad Zahid; Hassan, Mohamed M. M.; Qari, Sameer H. H.; Aziz, Muhammad Kashif; Khan, Zulqurnain
通讯作者	Khan, Z
来源期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
出版年	2022
卷号	13
英文摘要	Food security is facing a major threat from salinity and there is a need to develop salt tolerant crop varieties to ensure that the demand for food from the world's increasing population is met. Salinity mostly occurs in arid and semi-arid regions. It may cause many adverse physiological effects on plants, i.e., toxic ion accumulation, disturbed osmotic potential, and decreased crop yield. The present study aimed to investigate the morphological, physiological, biochemical, and genetic parameters of wheat genotypes under salt stress. Six wheat genotypes were screened for salt tolerance at the seedling and maturity stage. Seeds were sown at 0 and 150 mM of salinity level. Biochemical traits, i.e., shoot/root fresh and dry weight, chlorophyll a/b and total chlorophyll contents, shoot nitrogen, shoot phosphorus, proline, and carbohydrates were measured. Wheat genotypes showed a significant increase in free amino acids, shoot nitrogen, and total soluble proteins under saline conditions. Higher Na+/K+ ratio and free amino acids were estimated under 150 mM NaCl treatment in Pasban-90 and found to be the most salt-tolerant genotype. By contrast, reduced proline, total chlorophyll, and Na+/K+ ratio were found in Kohistan-97 marking it to be sensitive to stress. Expression analysis of HKTs genes was performed to validate the results of two contrasting genotypes. The differential expression of HKT2; 1 and HKT2; 3 explained the tissue and genotype specific epigenetic variations. Our findings indicated that these selected genotypes can be further used for molecular studies to find out QTLs/genes related to salinity. This suggests that, in contrasting wheat genotypes, there is a differentially induced defense response to salt stress, indicating a functional correlation between salt stress tolerance and differential expression pattern in wheat.
英文关键词	salt tolerance sodium chloride wheat yield expression analysis high affinity potassium transporters
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000854996400001
WOS关键词	SALINITY TOLERANCE ; HKT TRANSPORTERS ; STRESS ; GROWTH ; EXPRESSION ; RESISTANCE ; SEEDLINGS ; PLANTS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392831
推荐引用方式 GB/T 7714	Irshad, Ahsan,Ahmed, Rana Imtiaz,Rehman, Shoaib Ur,et al. Characterization of salt tolerant wheat genotypes by using morpho-physiological, biochemical, and molecular analysis[J],2022,13.
APA	Irshad, Ahsan.,Ahmed, Rana Imtiaz.,Rehman, Shoaib Ur.,Sun, Guozhong.,Ahmad, Furqan.,...&Khan, Zulqurnain.(2022).Characterization of salt tolerant wheat genotypes by using morpho-physiological, biochemical, and molecular analysis.FRONTIERS IN PLANT SCIENCE,13.
MLA	Irshad, Ahsan,et al."Characterization of salt tolerant wheat genotypes by using morpho-physiological, biochemical, and molecular analysis".FRONTIERS IN PLANT SCIENCE 13(2022).