干旱区生态环境知识资源中心(Arid): Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.]

Arid

DOI	10.3389/fpls.2022.962667
	Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.]
	Aher, Rasika Rajendra; Reddy, Palakolanu Sudhakar; Bhunia, Rupam Kumar; Flyckt, Kayla S.; Shankhapal, Aishwarya R.; Ojha, Rabishankar; Everard, John D.; Wayne, Laura L.; Ruddy, Brian M.; Deonovic, Benjamin; Gupta, Shashi K.; Sharma, Kiran K.; Bhatnagar-Mathur, Pooja
通讯作者	Bhatnagar-Mathur, P
来源期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
出版年	2022
卷号	13
英文摘要	Pearl millet is an important cereal crop of semi-arid regions since it is highly nutritious and climate resilient. However, pearl millet is underutilized commercially due to the rapid onset of hydrolytic rancidity of seed lipids post-milling. We investigated the underlying biochemical and molecular mechanisms of rancidity development in the flour from contrasting inbred lines under accelerated aging conditions. The breakdown of storage lipids (triacylglycerols; TAG) was accompanied by free fatty acid accumulation over the time course for all lines. The high rancidity tines had the highest amount of FFA by day 21, suggesting that TAG lipases may be the cause of rancidity. Additionally, the high rancidity tines manifested substantial amounts of volatile aldehyde compounds, which are characteristic products of lipid oxidation. Lipases with expression in seed post-milling were sequenced from low and high rancidity lines. Polymorphisms were identified in two TAG lipase genes (PgTAGLip1 and PgTAGLip2) from the low rancidity tine. Expression in a yeast model system confirmed these mutants were non-functional. We provide a direct mechanism to alleviate rancidity in pearl millet flour by identifying mutations in key TAG lipase genes that are associated with tow rancidity. These genetic variations can be exploited through molecular breeding or precision genome technologies to develop elite pearl millet cultivars with improved flour shelf life.
英文关键词	pearl millet Pennisetum glaucum (L.) R. Br. millet milled flour lipase triacylglycerol rancidity shelf life
类型	Article
语种	英语
开放获取类型	gold, Green Published, Green Accepted
收录类别	SCI-E
WOS记录号	WOS:000871903700001
WOS关键词	EXPRESSION ; YEAST
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392813
推荐引用方式 GB/T 7714	Aher, Rasika Rajendra,Reddy, Palakolanu Sudhakar,Bhunia, Rupam Kumar,et al. Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.][J],2022,13.
APA	Aher, Rasika Rajendra.,Reddy, Palakolanu Sudhakar.,Bhunia, Rupam Kumar.,Flyckt, Kayla S..,Shankhapal, Aishwarya R..,...&Bhatnagar-Mathur, Pooja.(2022).Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.].FRONTIERS IN PLANT SCIENCE,13.
MLA	Aher, Rasika Rajendra,et al."Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.]".FRONTIERS IN PLANT SCIENCE 13(2022).