干旱区生态环境知识资源中心(Arid): Genome-wide identification of XTH genes in Liriodendron chinense and functional characterization of LcXTH21

Arid

DOI	10.3389/fpls.2022.1014339
	Genome-wide identification of XTH genes in Liriodendron chinense and functional characterization of LcXTH21
	Wu, Junpeng; Zong, Yaxian; Tu, Zhonghua; Yang, Lichun; li, Wei; Cui, Zhengkun; Hao, Ziyuan; Li, Huogen
通讯作者	Li, HG
来源期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
出版年	2022
卷号	13
英文摘要	Liriodendron chinense is a relic tree species of the family Magnoliaceae with multiple uses in timber production, landscape decoration, and afforestation. L. chinense often experiences drought stress in arid areas. However, the molecular basis underlying the drought response of L. chinense remains unclear. Many studies have reported that the xyloglucan endotransglucosylase/hydrolase (XTH) family plays an important role in drought stress resistance. Hereby, to explore the drought resistance mechanism of L. chinense, we identify XTH genes on a genome-wide scale in L. chinense. A total of 27 XTH genes were identified in L. chinense, and these genes were classified into three subfamilies. Drought treatment and RT-qPCR analysis revealed that six LcXTH genes significantly responded to drought stress, especially LcXTH21. Hence, we cloned the LcXTH21 gene and overexpressed it in tobacco via gene transfer to analyze its function. The roots of transgenic plants were more developed than those of wild-type plants under different polyethylene glycol (PEG) concentration, and further RT-qPCR analysis showed that LcXTH21 highly expressed in root compared to aboveground organs, indicating that LcXTH21 may play a role in drought resistance through promoting root development. The results of this study provide new insights into the roles of LcXTH genes in the drought stress response. Our findings will also aid future studies of the molecular mechanisms by which LcXTH genes contribute to the drought response.
英文关键词	Liriodendron chinense XTH family drought stress response genome identification root development
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000884822600001
WOS关键词	XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE ; TRANSCRIPTION FACTOR ; KAPPA-CARRAGEENASE ; DROUGHT TOLERANCE ; ABIOTIC STRESS ; ABSCISIC-ACID ; GRAIN-YIELD ; EXPRESSION ; DIVERSITY ; EVOLUTION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392812
推荐引用方式 GB/T 7714	Wu, Junpeng,Zong, Yaxian,Tu, Zhonghua,et al. Genome-wide identification of XTH genes in Liriodendron chinense and functional characterization of LcXTH21[J],2022,13.
APA	Wu, Junpeng.,Zong, Yaxian.,Tu, Zhonghua.,Yang, Lichun.,li, Wei.,...&Li, Huogen.(2022).Genome-wide identification of XTH genes in Liriodendron chinense and functional characterization of LcXTH21.FRONTIERS IN PLANT SCIENCE,13.
MLA	Wu, Junpeng,et al."Genome-wide identification of XTH genes in Liriodendron chinense and functional characterization of LcXTH21".FRONTIERS IN PLANT SCIENCE 13(2022).