干旱区生态环境知识资源中心(Arid): Bacillus amyloliquefaciens Rescues Glycyrrhizic Acid Loss Under Drought Stress in Glycyrrhiza uralensis by Activating the Jasmonic Acid Pathway

Arid

DOI	10.3389/fmicb.2021.798525
	Bacillus amyloliquefaciens Rescues Glycyrrhizic Acid Loss Under Drought Stress in Glycyrrhiza uralensis by Activating the Jasmonic Acid Pathway
	Yue, Liang; Uwaremwe, Constantine; Tian, Yuan; Liu, Yang; Zhao, Xia; Zhou, Qin; Wang, Yun; Zhang, Yubao; Liu, Bailong; Cui, Zengtuan; Dun, Chengchao; Wang, Ruoyu
通讯作者	Wang, RY
来源期刊	FRONTIERS IN MICROBIOLOGY
EISSN	1664-302X
出版年	2022
卷号	12
英文摘要	Drought is a major factor limiting the production of the perennial medicinal plant Glycyrrhiza uralensis Fisch. (Fabaceae) in Northwest China. In this study, 1-year-old potted plants were inoculated with the strain Bacillus amyloliquefaciens FZB42, using a gradient of concentrations (CFU), to test for microbe-induced host tolerance to drought condition treatments in a greenhouse experiment. At the concentration of 10(8) CFU ml(-1), FZB42 had significant growth-promoting effect on G. uralensis: the root biomass was 1.52, 0.84, 0.94, and 0.38 times that under normal watering and mild, moderate, and severe drought stress conditions, respectively. Under moderate drought, the positive impact of FZB42 on G. uralensis growth was most pronounced, with both developing axial and lateral roots strongly associated with indoleacetic acid (IAA) accumulation. An untargeted metabolomic analysis and physiological measurements of mature roots revealed that FZB42 improved the antioxidant system of G. uralensis through the accumulation of proline and sucrose, two osmotic adjustment solutes, and by promoting catalase (CAT) activity under moderate drought stress. Furthermore, significantly higher levels of total flavonoids, liquiritin, and glycyrrhizic acid (GA), the pharmacologically active substances of G. uralensis, were found in the roots of inoculated plants after FZB42 inoculation under all imposed drought conditions. The jasmonic acid (JA) content, which is closely related to plant defense responses and secondary metabolites' production, was greatly increased in roots after the bacterial inoculations, indicating that FZB42 activated the JA pathway. Taken together, our results demonstrate that inoculation with FZB42 alleviates the losses in production and pharmacological metabolites of G. uralensis caused by drought via the JA pathway's activation. These results provide a developed prospect of a microbial agent to improve the yield and quality of medical plants in arid and semi-arid regions.
英文关键词	PGPR Glycyrrhiza uralensis glycyrrhizic acid (GA) drought jasmonic acid (JA)
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000778743300001
WOS关键词	GROWTH-PROMOTING RHIZOBACTERIA ; PLANT-GROWTH ; AZOSPIRILLUM INOCULATION ; ENDOPHYTIC COLONIZATION ; METABOLITE ACCUMULATION ; ANTIOXIDANT ENZYMES ; SPECTROMETRY DATA ; METHYL JASMONATE ; WATER-STRESS ; MECHANISM
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392776
推荐引用方式 GB/T 7714	Yue, Liang,Uwaremwe, Constantine,Tian, Yuan,et al. Bacillus amyloliquefaciens Rescues Glycyrrhizic Acid Loss Under Drought Stress in Glycyrrhiza uralensis by Activating the Jasmonic Acid Pathway[J],2022,12.
APA	Yue, Liang.,Uwaremwe, Constantine.,Tian, Yuan.,Liu, Yang.,Zhao, Xia.,...&Wang, Ruoyu.(2022).Bacillus amyloliquefaciens Rescues Glycyrrhizic Acid Loss Under Drought Stress in Glycyrrhiza uralensis by Activating the Jasmonic Acid Pathway.FRONTIERS IN MICROBIOLOGY,12.
MLA	Yue, Liang,et al."Bacillus amyloliquefaciens Rescues Glycyrrhizic Acid Loss Under Drought Stress in Glycyrrhiza uralensis by Activating the Jasmonic Acid Pathway".FRONTIERS IN MICROBIOLOGY 12(2022).