干旱区生态环境知识资源中心(Arid): Amanitins in Wild Mushrooms: The Development of HPLC-UV-EC and HPLC-DAD-MS Methods for Food Safety Purposes

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DOI	10.3390/foods11233929
	Amanitins in Wild Mushrooms: The Development of HPLC-UV-EC and HPLC-DAD-MS Methods for Food Safety Purposes
	Barbosa, Isabel; Domingues, Catia; Barbosa, Rui M.; Ramos, Fernando
通讯作者	Ramos, F
来源期刊	FOODS
EISSN	2304-8158
出版年	2022
卷号	11 期号:23
英文摘要	Mushroom poisoning remains a serious food safety and health concern in some parts of the world due to its morbidity and mortality. Identification of mushroom toxins at an early stage of suspected intoxication is crucial for a rapid therapeutic decision. In this study, a new extraction method was developed to determine alpha- and beta-amanitin in mushroom samples collected from central Portugal. High-performance liquid chromatography with in-line ultraviolet and electrochemical detection was implemented to improve the specificity of the method. The method was fully validated for linearity (0.5-20.0 mu g.mL(-1)), sensitivity, recovery, and precision based on a matrix-matched calibration method. The limit of detection was 55 mu g mL(-1) (UV) and 62 mu g mL(-1) (EC) for alpha-amanitin and 64 mu g mL(-1) (UV) and 24 mu g mL(-1) (EC) for beta-amanitin. Intra- and inter-day precision differences were less than 13%, and the recovery ratios ranged from 89% to 117%. The developed method was successfully applied to fourteen Amanita species (A. sp.) and compared with five edible mushroom samples after extraction with Oasis (R) PRIME HLB cartridges without the conditioning and equilibration step. The results revealed that the A. phalloides mushrooms present the highest content of alpha- and beta-amanitin, which is in line with the HPLC-DAD-MS. In sum, the developed analytical method could benefit food safety assessment and contribute to food-health security, as it is rapid, simple, sensitive, accurate, and selectively detects alpha- and beta-amanitin in any mushroom samples.
英文关键词	mushroom toxins alpha-amanitin beta-amanitin HPLC mass spectrometry
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000898574000001
WOS关键词	LIQUID-CHROMATOGRAPHIC DETERMINATION ; ALPHA-AMANITIN ; MASS-SPECTROMETRY ; BETA-AMANITIN ; TOXINS ; PHALLOIDES ; PHALLOTOXINS ; AMATOXINS ; QUANTIFICATION ; IDENTIFICATION
WOS类目	Food Science & Technology
WOS研究方向	Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392603
推荐引用方式 GB/T 7714	Barbosa, Isabel,Domingues, Catia,Barbosa, Rui M.,et al. Amanitins in Wild Mushrooms: The Development of HPLC-UV-EC and HPLC-DAD-MS Methods for Food Safety Purposes[J],2022,11(23).
APA	Barbosa, Isabel,Domingues, Catia,Barbosa, Rui M.,&Ramos, Fernando.(2022).Amanitins in Wild Mushrooms: The Development of HPLC-UV-EC and HPLC-DAD-MS Methods for Food Safety Purposes.FOODS,11(23).
MLA	Barbosa, Isabel,et al."Amanitins in Wild Mushrooms: The Development of HPLC-UV-EC and HPLC-DAD-MS Methods for Food Safety Purposes".FOODS 11.23(2022).